Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0014272
UniProt IDP31947
Primary gene name(s)SFN
Synonym gene name(s)HME1
Protein name14-3-3 protein sigma
Protein functionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. {ECO:0000269|PubMed:18382127}.; FUNCTION: p53-regulated inhibitor of G2/M progression. {ECO:0000269|PubMed:18382127}.
Subcellular locationCytoplasm. Nucleus {ECO:0000250}. Secreted. Note=May be secreted by a non-classical secretory pathway.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P31947
Gene Ontology
(Biological Process)
Complete annatation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
establishment of skin barrier [GO:0061436];
intrinsic apoptotic signaling pathway in response to DNA damage [GO:0008630];
keratinization [GO:0031424];
keratinocyte development [GO:0003334];
membrane organization [GO:0061024];
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0043154];
negative regulation of keratinocyte proliferation [GO:0010839];
negative regulation of protein kinase activity [GO:0006469];
positive regulation of cell growth [GO:0030307];
positive regulation of epidermal cell differentiation [GO:0045606];
positive regulation of protein export from nucleus [GO:0046827];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0000079];
regulation of epidermal cell division [GO:0010482];
release of cytochrome c from mitochondria [GO:0001836];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interaction109072
Phylogenetic treeP31947
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3unknownunknownunknown
AZA vs. DISUunknownunknownunknown
AZA vs. IL7unknownunknownunknown
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3unknownunknownunknown
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZAunknownunknownunknown
DMSO vs. CD3unknownunknownunknown
DMSO vs. DISUunknownunknownunknown
DMSO vs. IL7unknownunknownunknown
DMSO vs. SAHAunknownunknownunknown
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latencyunknownunknownunknown
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.157296 0.961466
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1YWT X-ray 2.4Å A/B=1-248.
1YZ5 X-ray 2.8Å A/B=1-248.
3IQJ X-ray 1.1Å A=1-231.
3IQU X-ray 1.0Å A=1-231.
3IQV X-ray 1.2Å A=1-231.
3LW1 X-ray 1.2Å A=1-248.
3MHR X-ray 1.1Å A=1-231.
3O8I X-ray 2.0Å A=1-231.
3P1N X-ray 1.4Å A=1-231.
3P1O X-ray 1.9Å A=1-231.
3P1P X-ray 1.9Å A=1-231.
3P1Q X-ray 1.7Å A=1-231.
3P1R X-ray 1.7Å A=1-231.
3P1S X-ray 1.6Å A=1-231.
3SMK X-ray 2.1Å A=1-231.
3SML X-ray 1.9Å A=1-231.
3SMM X-ray 2.0Å A=1-231.
3SMN X-ray 2.0Å A=1-231.
3SMO X-ray 1.8Å A=1-231.
3SP5 X-ray 1.8Å A=1-231.
3SPR X-ray 1.9Å A=1-231.
3T0L X-ray 1.6Å A=1-231.
3T0M X-ray 1.6Å A=1-231.
3U9X X-ray 1.8Å A=1-231.
3UX0 X-ray 1.7Å A=1-231.
4DAT X-ray 1.4Å A=1-231.
4DAU X-ray 2.0Å A=1-231.
4DHM X-ray 1.7Å A=1-231.
4DHN X-ray 1.8Å A=1-231.
4DHO X-ray 1.7Å A=1-231.
4DHP X-ray 1.7Å A=1-231.
4DHQ X-ray 1.7Å A=1-231.
4DHR X-ray 1.4Å A=1-231.
4DHS X-ray 1.7Å A=1-231.
4DHT X-ray 1.8Å A=1-231.
4DHU X-ray 1.6Å A=1-231.
4FL5 X-ray 1.9Å A/B=1-231.
4FR3 X-ray 1.9Å A=1-231.
4HQW X-ray 2.3Å A=1-231.
4HRU X-ray 3.1Å A=1-231.
4IEA X-ray 1.7Å A=1-231.
4JC3 X-ray 2.0Å A=1-231.
4JDD X-ray 2.1Å A=1-231.
4QLI X-ray 1.4Å A=1-231.
4Y32 X-ray 1.7Å A/B=1-231.
4Y3B X-ray 1.8Å A/B=1-231.
4Y5I X-ray 1.4Å A/B=1-231.
5BTV X-ray 1.7Å A=1-231.
5HF3 X-ray 1.8Å A=1-231.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04115 p53 signaling pathway - Homo sapiens (human)
hsa04960 Aldosterone-regulated sodium reabsorption - Homo sapiens (human)