Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0013607
UniProt IDP25942
Primary gene name(s)CD40
Synonym gene name(s)TNFRSF5
Protein nameTumor necrosis factor receptor superfamily member 5
Protein functionReceptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Subcellular locationIsoform I: Cell membrane;
Single-pass type I membrane protein.;
SUBCELLULAR LOCATION: Isoform II: Secreted.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P25942
Gene Ontology
(Biological Process)
Complete annatation
apoptotic signaling pathway [GO:0097190];
B cell proliferation [GO:0042100];
cellular calcium ion homeostasis [GO:0006874];
cellular response to lipopolysaccharide [GO:0071222];
cellular response to mechanical stimulus [GO:0071260];
defense response to protozoan [GO:0042832];
defense response to virus [GO:0051607];
immune response [GO:0006955];
immune response-regulating cell surface receptor signaling pathway [GO:0002768];
inflammatory response [GO:0006954];
multicellular organism development [GO:0007275];
platelet activation [GO:0030168];
positive regulation of B cell proliferation [GO:0030890];
positive regulation of endothelial cell apoptotic process [GO:2000353];
positive regulation of GTPase activity [GO:0043547];
positive regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043123];
positive regulation of interleukin-12 production [GO:0032735];
positive regulation of isotype switching to IgG isotypes [GO:0048304];
positive regulation of MAP kinase activity [GO:0043406];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
positive regulation of protein kinase C signaling [GO:0090037];
positive regulation of protein phosphorylation [GO:0001934];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of tyrosine phosphorylation of Stat1 protein [GO:0042511];
protein complex assembly [GO:0006461];
protein kinase B signaling [GO:0043491];
regulation of cell proliferation [GO:0042127];
regulation of immune response [GO:0050776];
regulation of immunoglobulin secretion [GO:0051023];
response to lipopolysaccharide [GO:0032496];
tumor necrosis factor-mediated signaling pathway [GO:0033209]
Gene Ontology
(Molecular Function)
Complete annatation
antigen binding [GO:0003823];
enzyme binding [GO:0019899];
receptor activity [GO:0004872];
signal transducer activity [GO:0004871];
tumor necrosis factor-activated receptor activity [GO:0005031];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
CD40 receptor complex [GO:0035631];
cell surface [GO:0009986];
cytoplasm [GO:0005737];
external side of plasma membrane [GO:0009897];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
integral component of plasma membrane [GO:0005887];
intracellular membrane-bounded organelle [GO:0043231];
plasma membrane [GO:0005886]
Protein-protein interaction107396
Phylogenetic treeP25942
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3unknownunknownunknown
AZA vs. DISUunknownunknownunknown
AZA vs. IL7unknownunknownunknown
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3unknownunknownunknown
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZAunknownunknownunknown
DMSO vs. CD3unknownunknownunknown
DMSO vs. DISUunknownunknownunknown
DMSO vs. IL7unknownunknownunknown
DMSO vs. SAHAunknownunknownunknown
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latencyunknownunknownunknown
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.931033 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1CDF Model - A=24-144.
1CZZ X-ray 2.7Å D/E=250-258.
1D00 X-ray 2.0Å I/J/K/L/M/N/O/P=250-254.
1FLL X-ray 3.5Å X/Y=246-266.
1LB6 X-ray 1.8Å B=230-236.
3QD6 X-ray 3.5Å R/S/T/U=21-190.
5DMI X-ray 3.6Å A=23-193.
5DMJ X-ray 2.7Å A/D/F=23-193.
5IHL X-ray 3.3Å A/D/F/H=23-193.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 downregulates 23100517
Vpu upregulates 15078922
20012522
21922073
matrix upregulates 22558273
Vpr downregulates 15611322
19275586
19458171
Tat upregulates 11751963
17312171
25636783
Envelope surface glycoprotein gp120 upregulates 15749123
24632372
Pr55(Gag) interacts with 21239998
Envelope surface glycoprotein gp120 inhibits 11087201
7541827
17918746

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04060 Cytokine-cytokine receptor interaction - Homo sapiens (human)
hsa04064 NF-kappa B signaling pathway - Homo sapiens (human)
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa04620 Toll-like receptor signaling pathway - Homo sapiens (human)
hsa04672 Intestinal immune network for IgA production - Homo sapiens (human)
hsa05144 Malaria - Homo sapiens (human)
hsa05145 Toxoplasmosis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05202 Transcriptional misregulation in cancer - Homo sapiens (human)
hsa05310 Asthma - Homo sapiens (human)
hsa05320 Autoimmune thyroid disease - Homo sapiens (human)
hsa05322 Systemic lupus erythematosus - Homo sapiens (human)
hsa05330 Allograft rejection - Homo sapiens (human)
hsa05340 Primary immunodeficiency - Homo sapiens (human)
hsa05416 Viral myocarditis - Homo sapiens (human)
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