Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0013402
UniProt IDQ9ULV1
Primary gene name(s)FZD4
Synonym gene name(s)unknown
Protein nameFrizzled-4
Protein functionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin, CTNNB1 canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, CTNNB1 and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin, NDP. In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin, NDP, promoting in both cases beta-catenin, CTNNB1 accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Subcellular locationMembrane;
Multi-pass membrane protein. Cell membrane {ECO:0000250};
Multi-pass membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9ULV1
Gene Ontology
(Biological Process)
Complete annatation
canonical Wnt signaling pathway [GO:0060070];
cellular response to retinoic acid [GO:0071300];
cerebellum vasculature morphogenesis [GO:0061301];
extracellular matrix-cell signaling [GO:0035426];
locomotion involved in locomotory behavior [GO:0031987];
negative regulation of cell-substrate adhesion [GO:0010812];
neuron differentiation [GO:0030182];
positive regulation of JUN kinase activity [GO:0043507];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
positive regulation of transcription, DNA-templated [GO:0045893];
progesterone secretion [GO:0042701];
regulation of vascular endothelial growth factor receptor signaling pathway [GO:0030947];
retinal blood vessel morphogenesis [GO:0061304];
retina vasculature morphogenesis in camera-type eye [GO:0061299];
sensory perception of sound [GO:0007605];
substrate adhesion-dependent cell spreading [GO:0034446];
vasculogenesis [GO:0001570];
Wnt signaling pathway [GO:0016055];
Wnt signaling pathway, calcium modulating pathway [GO:0007223];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
cytokine binding [GO:0019955];
G-protein coupled receptor activity [GO:0004930];
PDZ domain binding [GO:0030165];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803];
ubiquitin protein ligase binding [GO:0031625];
Wnt-activated receptor activity [GO:0042813];
Wnt-protein binding [GO:0017147]
Gene Ontology
(Cellular Component)
Complete annatation
cell-cell junction [GO:0005911];
cell surface [GO:0009986];
clathrin-coated endocytic vesicle membrane [GO:0030669];
extracellular exosome [GO:0070062];
integral component of membrane [GO:0016021];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886]
Protein-protein interaction113918
Phylogenetic treeQ9ULV1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3unknownunknownunknown
AZA vs. DISUunknownunknownunknown
AZA vs. IL7unknownunknownunknown
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3unknownunknownunknown
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZAunknownunknownunknown
DMSO vs. CD3unknownunknownunknown
DMSO vs. DISUunknownunknownunknown
DMSO vs. IL7unknownunknownunknown
DMSO vs. SAHAunknownunknownunknown
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latency-0.09432771697677940.8854377958725540.999834320637052
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
NOTEST 2.55714 1
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5BPB X-ray 2.2Å A/B/C/D=42-179.
5BPQ X-ray 2.4Å A/B/C/D=42-179.
5BQC X-ray 3.0Å B=42-179.
5BQE X-ray 2.3Å C=42-179.
5CL1 X-ray 3.8Å C/D=38-160.
5CM4 X-ray 2.4Å A/B=40-164.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04150 mTOR signaling pathway - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04550 Signaling pathways regulating pluripotency of stem cells - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05217 Basal cell carcinoma - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)
Menu