Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012817
UniProt IDO43462
Primary gene name(s)MBTPS2
Synonym gene name(s)S2P
Protein nameMembrane-bound transcription factor site-2 protease
Protein functionIntramembrane proteolysis of sterol-regulatory element-binding proteins, SREBPs within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.
Subcellular locationMembrane {ECO:0000305|PubMed:19361614};
Multi-pass membrane protein {ECO:0000305|PubMed:19361614}. Cytoplasm {ECO:0000269|PubMed:19361614}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43462
Gene Ontology
(Biological Process)
Complete annatation
ATF6-mediated unfolded protein response [GO:0036500];
cholesterol metabolic process [GO:0008203];
membrane protein intracellular domain proteolysis [GO:0031293];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress [GO:1990440];
response to endoplasmic reticulum stress [GO:0034976]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
metalloendopeptidase activity [GO:0004222];
metallopeptidase activity [GO:0008237]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021]
Protein-protein interaction119495
Phylogenetic treeO43462
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.25951074029990.0001606694290751110.000733821101521234
AZA vs. DISU-0.4231336087694120.1002414376466430.641672647375295
AZA vs. IL70.1392892070535250.4786861452730910.999311006273513
AZA vs. SAHA-0.1919993274635190.4399430700652570.787415175806649
DISU vs. CD3-1.69473203729446.05515831009207e-064.7738209396615e-05
DISU vs. IL70.5531246038488130.03102367182172520.227804117249151
DISU vs. SAHA0.2318145383317250.4355016369331730.781929674836169
DMSO vs. AZA-0.07100066962130980.6817719544901891
DMSO vs. CD3-1.340824733439153.9983252422493e-050.000198860250163373
DMSO vs. DISU0.3504412463752330.1580879779855310.663602764918316
DMSO vs. IL70.2176060385255770.2374653224367120.743226205962435
DMSO vs. SAHA-0.1281131726421680.5936450299098550.862163679594325
HIV vs. Mock in Activation0.01497300841473460.9808328729844260.999983755607037
HIV vs. Mock in Latency0.09896943631768380.5585324261616840.999834320637052
IL7 vs. CD3-1.112354134202510.0006451428463318810.00291756319314745
SAHA vs. CD3-1.476870389297854.44800854420002e-050.000235198206179278
SAHA vs. IL7-0.3355654077602940.175219680412610.401091610184949
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0270695 0.90686
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)