Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012805
UniProt IDO15344
Primary gene name(s)MID1
Synonym gene name(s)FXY, RNF59, TRIM18, XPRF
Protein nameE3 ubiquitin-protein ligase Midline-1
Protein functionHas E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. {ECO:0000269|PubMed:10400985, ECO:0000269|PubMed:11685209, ECO:0000269|PubMed:22613722}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:10077590}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:10077590}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:10077590}. Note=Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O15344
Gene Ontology
(Biological Process)
Complete annatation
interferon-gamma-mediated signaling pathway [GO:0060333];
microtubule cytoskeleton organization [GO:0000226];
negative regulation of microtubule depolymerization [GO:0007026];
pattern specification process [GO:0007389];
positive regulation of stress-activated MAPK cascade [GO:0032874];
protein localization to microtubule [GO:0035372]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
ligase activity [GO:0016874];
microtubule binding [GO:0008017];
phosphoprotein binding [GO:0051219];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803];
ubiquitin protein ligase binding [GO:0031625];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
microtubule [GO:0005874];
microtubule associated complex [GO:0005875];
spindle [GO:0005819]
Protein-protein interaction110427
Phylogenetic treeO15344
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.640065667648563.18479001304439e-050.000175571159753493
AZA vs. DISU-0.1144712586142090.843319466697360.987278474758931
AZA vs. IL7-0.6452706294323980.1728644691632260.941150532286992
AZA vs. SAHA0.1536548321190580.74880717994950.931215822620618
DISU vs. CD32.510946771135040.0004285900721023860.00200203493860284
DISU vs. IL7-0.5387035982214040.3195969077584660.701400987692369
DISU vs. SAHA0.2708418400694140.6187740603360750.879584037336788
DMSO vs. AZA0.1806436908658760.7081417257309921
DMSO vs. CD32.801256333434421.24814850521737e-068.79483500051437e-06
DMSO vs. DISU0.2928471366182650.5910617441088910.935856053607981
DMSO vs. IL7-0.8171649334880240.05596028674305350.463763012974044
DMSO vs. SAHA-0.03222320660829940.9405015837816980.985969627794517
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latency0.2009651867867190.6844589859310890.999834320637052
IL7 vs. CD31.999684759244960.0003894320266374370.00188682383076377
SAHA vs. CD32.765234757785051.69889652279487e-061.30424710129998e-05
SAHA vs. IL70.7925115378609120.06410036556044640.216676342015014
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.66538 0.00475227
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DQ5 NMR - A=168-214.
2FFW NMR - A=87-164.
2JUN NMR - A=114-214.
5IM8 NMR - A=320-379.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)