Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012657
UniProt IDP42772
Primary gene name(s)CDKN2B
Synonym gene name(s)MTS2
Protein nameCyclin-dependent kinase 4 inhibitor B
Protein functionInteracts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
Subcellular locationCytoplasm {ECO:0000269|PubMed:9230210}. Note=Also found in the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P42772
Gene Ontology
(Biological Process)
Complete annatation
cell cycle arrest [GO:0007050];
cellular response to extracellular stimulus [GO:0031668];
cellular response to nutrient [GO:0031670];
G2/M transition of mitotic cell cycle [GO:0000086];
megakaryocyte differentiation [GO:0030219];
mitotic cell cycle checkpoint [GO:0007093];
negative regulation of cell proliferation [GO:0008285];
negative regulation of epithelial cell proliferation [GO:0050680];
negative regulation of G1/S transition of mitotic cell cycle [GO:2000134];
negative regulation of phosphorylation [GO:0042326];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of transforming growth factor beta receptor signaling pathway [GO:0030511];
regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0000079];
spleen development [GO:0048536]
Gene Ontology
(Molecular Function)
Complete annatation
cyclin-dependent protein serine/threonine kinase inhibitor activity [GO:0004861];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction107464
Phylogenetic treeP42772
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.56467259062532.85133978983332e-072.49421899665512e-06
AZA vs. DISU-0.1768733475135310.6359848521799690.960057478314726
AZA vs. IL70.478660594955570.05693864726971410.69101145756554
AZA vs. SAHA0.09694516805718450.7328788041258640.925643771606674
DISU vs. CD32.376607457338921.81327247582574e-050.000126075965408659
DISU vs. IL70.6452227801877510.06179102069930560.327682641692784
DISU vs. SAHA0.2769696120606420.4531695579993190.794338033101621
DMSO vs. AZA-0.001619013902447580.9961646167446231
DMSO vs. CD32.560956412779012.23521490860801e-061.48931944264395e-05
DMSO vs. DISU0.1762072780419470.6704754206414870.954293973277976
DMSO vs. IL70.4862060590279170.113974750148990.601912495801813
DMSO vs. SAHA0.09139368675575130.7861987903908080.939630662728404
HIV vs. Mock in Activation1.080081837591110.1002131894809020.999983755607037
HIV vs. Mock in Latency-0.06586021851424740.9410037299353170.999834320637052
IL7 vs. CD33.046753390919992.22996066057135e-104.45194662738967e-09
SAHA vs. CD32.639887382512859.32182104840251e-089.85823461048603e-07
SAHA vs. IL7-0.3826135476329050.1166298987687160.314999975356548
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.519059 0.496592
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04110 Cell cycle - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05222 Small cell lung cancer - Homo sapiens (human)