Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012592
UniProt IDQ96A56
Primary gene name(s)TP53INP1
Synonym gene name(s)P53DINP1, SIP
Protein nameTumor protein p53-inducible nuclear protein 1
Protein functionAntiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species, ROS regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. {ECO:0000269|PubMed:11511362, ECO:0000269|PubMed:22421968, ECO:0000269|PubMed:22470510}.
Subcellular locationCytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Note=Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96A56
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
autophagic cell death [GO:0048102];
autophagosome assembly [GO:0000045];
cell cycle arrest [GO:0007050];
cellular response to ethanol [GO:0071361];
cellular response to hydroperoxide [GO:0071447];
cellular response to methyl methanesulfonate [GO:0072703];
cellular response to UV [GO:0034644];
negative regulation of cell migration [GO:0030336];
negative regulation of cell proliferation [GO:0008285];
negative regulation of fibroblast proliferation [GO:0048147];
negative regulation of gene expression [GO:0010629];
negative regulation of myofibroblast differentiation [GO:1904761];
positive regulation of apoptotic signaling pathway [GO:2001235];
positive regulation of autophagy [GO:0010508];
positive regulation of transcription, DNA-templated [GO:0045893];
regulation of apoptotic process [GO:0042981];
regulation of signal transduction by p53 class mediator [GO:1901796];
response to heat [GO:0009408];
response to stress [GO:0006950];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
antioxidant activity [GO:0016209]
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome [GO:0005776];
cytoplasm [GO:0005737];
cytoplasmic vesicle [GO:0031410];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction125152
Phylogenetic treeQ96A56
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.542980934130322.4580337765201e-136.49955878591419e-12
AZA vs. DISU0.9935920965783530.01132666395248420.259413760018377
AZA vs. IL7-0.749509543940690.0001143357187807230.0169548752810249
AZA vs. SAHA0.3142423515254020.1990933732653760.566502890178473
DISU vs. CD33.521833818467023.33066907387547e-151.81907629056517e-13
DISU vs. IL7-1.751674430812247.91168759939342e-060.000731709758042061
DISU vs. SAHA-0.6787626046003240.1091739481657920.424062003300682
DMSO vs. AZA-0.1762132104037180.2943360675412831
DMSO vs. CD32.352413071892182.93454149868921e-125.96926901370653e-11
DMSO vs. DISU-1.172287871561720.002439427270667150.104437980025437
DMSO vs. IL7-0.5660016079702890.00182682202493090.0826040307465751
DMSO vs. SAHA0.4833531407500690.04110232292677650.225665977715646
HIV vs. Mock in Activation0.6579948405857970.2952601651176330.999983755607037
HIV vs. Mock in Latency-0.0162675922861550.9303589288916110.999834320637052
IL7 vs. CD31.801794914368135.80555573614916e-087.49846971816421e-07
SAHA vs. CD32.830765322985149.69224700497762e-143.54519811565565e-12
SAHA vs. IL71.058481742699442.03621291358091e-050.000549085392832177
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.587862 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05166 HTLV-I infection - Homo sapiens (human)