Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012232
UniProt IDP13765
Primary gene name(s)HLA-DOB
Synonym gene name(s)unknown
Protein nameHLA class II histocompatibility antigen, DO beta chain
Protein functionImportant modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.
Subcellular locationEndosome membrane;
Single-pass type I membrane protein. Lysosome membrane;
Single-pass type I membrane protein. Note=Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P13765
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
immune response [GO:0006955];
negative regulation of antigen processing and presentation of peptide antigen via MHC class II [GO:0002587]
Gene Ontology
(Molecular Function)
Complete annatation
MHC class II protein complex binding [GO:0023026];
MHC class II receptor activity [GO:0032395]
Gene Ontology
(Cellular Component)
Complete annatation
endosome membrane [GO:0010008];
integral component of membrane [GO:0016021];
lysosomal membrane [GO:0005765];
lysosome [GO:0005764];
MHC class II protein complex [GO:0042613]
Protein-protein interaction109357
Phylogenetic treeP13765
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5676575990961320.1190047325563110.202305360826609
AZA vs. DISU0.2264340828840140.4612218247781350.924263941482596
AZA vs. IL70.6289388669550260.01338348665265740.384246274886677
AZA vs. SAHA0.09206709538893540.763454569936730.936905231418095
DISU vs. CD3-0.3589722240073390.3619313128915610.495887348711816
DISU vs. IL70.4003130390552970.1744701847086830.54076187611732
DISU vs. SAHA-0.1304100959555370.6998550850931950.910966306316983
DMSO vs. AZA0.09742889877230690.6962425421889611
DMSO vs. CD3-0.4869189246194430.1670557379932210.258839318406652
DMSO vs. DISU-0.1306673179205570.6565674906756410.950002654032543
DMSO vs. IL70.5401038019321740.02643669630991940.330889922759818
DMSO vs. SAHA-0.01073568543571050.9706436230436480.993292390076406
HIV vs. Mock in Activation-0.3698585732093150.5774298515892210.999983755607037
HIV vs. Mock in Latency-0.5046557307481650.02037379480593350.531340108831559
IL7 vs. CD30.06958557614152240.8419662536921180.89826656363344
SAHA vs. CD3-0.5002702295227930.1946760531034580.295981448776141
SAHA vs. IL7-0.5394154293012890.06382307145802070.215952632807678
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.543687 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4I0P X-ray 3.2Å D/H=30-218.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) upregulates 18945465
Envelope surface glycoprotein gp120 downregulates 24162774
Envelope transmembrane glycoprotein gp41 incorporates 10729169
Envelope surface glycoprotein gp120 interacts with 1869305
Envelope surface glycoprotein gp160; precursor associates with 3489470
Nef inhibits 11593029
Nef downregulates 12970439
Pr55(Gag) regulated by 22860026
Tat downregulates 10661406
Vpu modulates 17959659
Envelope transmembrane glycoprotein gp41 upregulates 8084338
Envelope surface glycoprotein gp120 inhibits 2543930

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04145 Phagosome - Homo sapiens (human)
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
hsa04640 Hematopoietic cell lineage - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04672 Intestinal immune network for IgA production - Homo sapiens (human)
hsa04940 Type I diabetes mellitus - Homo sapiens (human)
hsa05140 Leishmaniasis - Homo sapiens (human)
hsa05145 Toxoplasmosis - Homo sapiens (human)
hsa05150 Staphylococcus aureus infection - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05168 Herpes simplex infection - Homo sapiens (human)
hsa05310 Asthma - Homo sapiens (human)
hsa05320 Autoimmune thyroid disease - Homo sapiens (human)
hsa05321 Inflammatory bowel disease (IBD) - Homo sapiens (human)
hsa05322 Systemic lupus erythematosus - Homo sapiens (human)
hsa05323 Rheumatoid arthritis - Homo sapiens (human)
hsa05330 Allograft rejection - Homo sapiens (human)
hsa05332 Graft-versus-host disease - Homo sapiens (human)
hsa05416 Viral myocarditis - Homo sapiens (human)