Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012108
UniProt IDQ8WWN8
Primary gene name(s)ARAP3
Synonym gene name(s)CENTD3
Protein nameArf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
Protein functionPhosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4,5P3 binding. Can be activated by phosphatidylinositol 3,4-bisphosphate, PtdIns(3,4,5P2 binding, albeit with lower efficiency. Acts on ARF6, RAC1, RHOA and CDC42. Plays a role in the internalization of anthrax toxin. {ECO:0000269|PubMed:11804589, ECO:0000269|PubMed:15569923}.
Subcellular locationCytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Cell projection, lamellipodium {ECO:0000250}. Cell projection, ruffle {ECO:0000250}. Note=Cytoplasmic, and associated with F-actin-rich membrane ruffles and lamellipodia. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8WWN8
Gene Ontology
(Biological Process)
Complete annatation
cytoskeleton organization [GO:0007010];
negative regulation of cell migration [GO:0030336];
negative regulation of Rac protein signal transduction [GO:0035021];
negative regulation of Rho protein signal transduction [GO:0035024];
regulation of cell shape [GO:0008360];
regulation of small GTPase mediated signal transduction [GO:0051056];
signal transduction [GO:0007165];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
metal ion binding [GO:0046872];
phosphatidylinositol-3,4,5-trisphosphate binding [GO:0005547];
phosphatidylinositol-3,4-bisphosphate binding [GO:0043325]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
lamellipodium [GO:0030027];
plasma membrane [GO:0005886];
ruffle [GO:0001726]
Protein-protein interaction122163
Phylogenetic treeQ8WWN8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1907125608544780.5758363882643940.683939155403479
AZA vs. DISU0.8772974563440120.001045790774940710.0792173373942529
AZA vs. IL7-0.8143694937883490.0002648424383421280.0307074621010069
AZA vs. SAHA0.6239549786376960.01650415926780870.135317815754642
DISU vs. CD31.053535311620220.005013559541690470.0162490639714991
DISU vs. IL7-1.705330784649314.30415147967267e-101.70750219884383e-07
DISU vs. SAHA-0.2510382411775240.4038079466027470.76259639186231
DMSO vs. AZA0.02979124075687890.8783114600682491
DMSO vs. CD30.2075378932201560.5325454661078750.638401961240959
DMSO vs. DISU-0.8497294232794270.0009518705839860480.0638470966353906
DMSO vs. IL7-0.836005189966326.66638027996269e-050.00889342324959625
DMSO vs. SAHA0.5889822761881910.01882728306856980.136463582338732
HIV vs. Mock in Activation0.5771555479133180.3693342384072050.999983755607037
HIV vs. Mock in Latency0.4798323286174290.1856579373840830.999834320637052
IL7 vs. CD3-0.6236548061720980.06621749814958410.137656404451432
SAHA vs. CD30.7914169225935910.03012181997014670.0650524257595459
SAHA vs. IL71.436927731418756.84266954209534e-084.84255402241996e-06
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.872643 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KG5 NMR - A=1-80.
2LNW NMR - B=1404-1412.
5JCP X-ray 2.1Å A/B=906-1107.
5JD0 X-ray 2.3Å A/B=906-1107.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)