Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0012045
UniProt IDQ13216
Primary gene name(s)ERCC8
Synonym gene name(s)CKN1, CSA
Protein nameDNA excision repair protein ERCC-8
Protein functionSubstrate-recognition component of the CSA complex, a DCX, DDB1-CUL4-X-box E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex, DCX(ERCC8 complex promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. {ECO:0000269|PubMed:16751180, ECO:0000269|PubMed:16916636, ECO:0000269|PubMed:16964240}.
Subcellular locationNucleus {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13216
Gene Ontology
(Biological Process)
Complete annatation
cellular response to DNA damage stimulus [GO:0006974];
nucleotide-excision repair [GO:0006289];
positive regulation of DNA repair [GO:0045739];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein autoubiquitination [GO:0051865];
protein polyubiquitination [GO:0000209];
response to oxidative stress [GO:0006979];
response to UV [GO:0009411];
response to X-ray [GO:0010165];
transcription-coupled nucleotide-excision repair [GO:0006283]
Gene Ontology
(Molecular Function)
Complete annatation
protein complex binding [GO:0032403]
Gene Ontology
(Cellular Component)
Complete annatation
Cul4A-RING E3 ubiquitin ligase complex [GO:0031464];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleotide-excision repair complex [GO:0000109];
nucleus [GO:0005634];
protein complex [GO:0043234]
Protein-protein interaction107581
Phylogenetic treeQ13216
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6796921711646010.04072723322945790.0840187324437982
AZA vs. DISU-0.3209018858613080.2180169125874730.806673279416684
AZA vs. IL70.1778733362236240.3747531317350420.999311006273513
AZA vs. SAHA-0.08516656545135230.7345036972221170.926519559157004
DISU vs. CD3-1.012628228234880.006221507313053380.0195096736502947
DISU vs. IL70.4897680951934360.05846581260576160.319106489873952
DISU vs. SAHA0.2364746272504110.4276258342152910.776911552450292
DMSO vs. AZA-0.09797606101172170.5822781602491571
DMSO vs. CD3-0.7877406024448930.01532993955708890.0357722991993624
DMSO vs. DISU0.2213378288508050.3779892203482190.856328555419071
DMSO vs. IL70.2825306523618280.1329985394018620.631680208406765
DMSO vs. SAHA0.005044073587791570.98341780838280.995773299525476
HIV vs. Mock in Activation-0.2286781377506520.7148580825960360.999983755607037
HIV vs. Mock in Latency0.02937754682739370.8662621931116760.999834320637052
IL7 vs. CD3-0.4920285691032840.1298872454293210.231746028687991
SAHA vs. CD3-0.7896029742826450.0275686546407480.0603076826006782
SAHA vs. IL7-0.2672399907087880.2848315298025020.53250036961961
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.317424 0.0740463
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4A11 X-ray 3.3Å B=1-396.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03420 Nucleotide excision repair - Homo sapiens (human)
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
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