Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0011999
UniProt IDQ969H0
Primary gene name(s)FBXW7
Synonym gene name(s)FBW7, FBX30, SEL10
Protein nameF-box/WD repeat-containing protein 7
Protein functionSubstrate recognition component of an SCF, SKP1-CUL1-F-box protein E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination, PubMed:17434132. Identified substrates include cyclin-E, CCNE1 or CCNE2, JUN, MYC, NOTCH1 released notch intracellular domain, NICD, and probably PSEN1, PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation, PubMed:14739463. {ECO:0000269|PubMed:11565034, ECO:0000269|PubMed:11585921, ECO:0000269|PubMed:14739463, ECO:0000269|PubMed:15103331, ECO:0000269|PubMed:17434132, ECO:0000269|PubMed:17558397, ECO:0000269|PubMed:17873522, ECO:0000269|PubMed:22608923, ECO:0000305|PubMed:12354302}.
Subcellular locationIsoform 1: Nucleus, nucleoplasm {ECO:0000269|PubMed:17558397}.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm {ECO:0000269|PubMed:17558397}.;
SUBCELLULAR LOCATION: Isoform 3: Nucleus, nucleolus {ECO:0000269|PubMed:17558397}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q969H0
Gene Ontology
(Biological Process)
Complete annatation
cellular response to DNA damage stimulus [GO:0006974];
cellular response to UV [GO:0034644];
lipid homeostasis [GO:0055088];
negative regulation of DNA endoreduplication [GO:0032876];
negative regulation of hepatocyte proliferation [GO:2000346];
negative regulation of Notch signaling pathway [GO:0045746];
negative regulation of SREBP signaling pathway [GO:2000639];
negative regulation of triglyceride biosynthetic process [GO:0010868];
positive regulation of epidermal growth factor-activated receptor activity [GO:0045741];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [GO:1903378];
positive regulation of proteasomal protein catabolic process [GO:1901800];
positive regulation of protein targeting to mitochondrion [GO:1903955];
positive regulation of protein ubiquitination [GO:0031398];
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:2000060];
positive regulation of ubiquitin-protein transferase activity [GO:0051443];
protein polyubiquitination [GO:0000209];
protein stabilization [GO:0050821];
protein ubiquitination [GO:0016567];
regulation of cell cycle G1/S phase transition [GO:1902806];
regulation of lipid storage [GO:0010883];
regulation of mitophagy [GO:1903146];
regulation of protein localization [GO:0032880];
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [GO:0031146];
sister chromatid cohesion [GO:0007062];
vasculature development [GO:0001944];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
cyclin binding [GO:0030332];
identical protein binding [GO:0042802];
phosphothreonine binding [GO:0050816];
protein binding, bridging [GO:0030674];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activator activity [GO:0097027];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
Parkin-FBXW7-Cul1 ubiquitin ligase complex [GO:1990452];
protein complex [GO:0043234];
SCF ubiquitin ligase complex [GO:0019005]
Protein-protein interaction120581
Phylogenetic treeQ969H0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.334556134757440.308306215584910.427189893636978
AZA vs. DISU0.2363166829603660.3554803710779480.891144071056453
AZA vs. IL70.07545884487007160.6970790111398350.999311006273513
AZA vs. SAHA0.3171687038806360.1967183043479670.564242642977567
DISU vs. CD3-0.1116417265923090.7611261981789380.834340652927425
DISU vs. IL7-0.169133505478680.5057110552370920.830044367655729
DISU vs. SAHA0.08185416258019980.7827308520675220.940637642254177
DMSO vs. AZA0.004497425287283180.9788375924532251
DMSO vs. CD3-0.3414265959183030.2874512584441380.397034309018147
DMSO vs. DISU-0.2335384678038020.3427263272124910.839767273617644
DMSO vs. IL70.07823074508884450.6661782187805990.931410444032928
DMSO vs. SAHA0.3052882318650730.1990248936247330.538124178654172
HIV vs. Mock in Activation-0.07815899407231270.9001586953551360.999983755607037
HIV vs. Mock in Latency0.07921020671038810.633735770790760.999834320637052
IL7 vs. CD3-0.2503618111467810.4372109564646360.572031967792236
SAHA vs. CD3-0.0427566119849730.9037343036925270.933530465189279
SAHA vs. IL70.2374815390743590.3330167546761390.5839802885049
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.142741 0.562499
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2OVP X-ray 2.9Å B=263-707.
2OVQ X-ray 2.6Å B=263-707.
2OVR X-ray 2.5Å B=263-707.
5IBK X-ray 2.5Å B/E=263-323.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)