Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0011615
UniProt IDP53675
Primary gene name(s)CLTCL1
Synonym gene name(s)CLH22, CLTCL, CLTD
Protein nameClathrin heavy chain 2
Protein functionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network, By similarity. {ECO:0000250}.
Subcellular locationCytoplasmic vesicle membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}. Membrane, coated pit {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}. Note=Cytoplasmic face of coated pits and vesicles. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P53675
Gene Ontology
(Biological Process)
Complete annatation
anatomical structure morphogenesis [GO:0009653];
intracellular protein transport [GO:0006886];
mitotic nuclear division [GO:0007067];
positive regulation of glucose import [GO:0046326];
receptor-mediated endocytosis [GO:0006898];
retrograde transport, endosome to Golgi [GO:0042147]
Gene Ontology
(Molecular Function)
Complete annatation
signal transducer activity [GO:0004871];
structural molecule activity [GO:0005198]
Gene Ontology
(Cellular Component)
Complete annatation
clathrin-coated vesicle [GO:0030136];
clathrin coat of trans-Golgi network vesicle [GO:0030130];
coated pit [GO:0005905];
coated vesicle [GO:0030135];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
late endosome [GO:0005770];
membrane [GO:0016020];
sorting endosome [GO:0097443];
spindle [GO:0005819];
trans-Golgi network [GO:0005802]
Protein-protein interaction113854
Phylogenetic treeP53675
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1718184558578140.6376481232013580.736065621344568
AZA vs. DISU0.5003107587877170.09166761701214390.623651930546018
AZA vs. IL7-0.2574782995780620.3296356881898530.999311006273513
AZA vs. SAHA1.5812741320722.26146540382999e-084.42718564900432e-06
DISU vs. CD30.3176102732444670.4154907076655840.548259644957612
DISU vs. IL7-0.7715798957104630.009224234264416760.111790613085444
DISU vs. SAHA1.084757895747630.0005839380310823650.0139028911002248
DMSO vs. AZA0.2855451184695560.2294417011138811
DMSO vs. CD30.1021005463163880.7711946671414670.836974833260299
DMSO vs. DISU-0.2185362460212590.4370663202644550.882337376789529
DMSO vs. IL7-0.5337718225550210.028365559398140.343738591581158
DMSO vs. SAHA1.292807266770311.37984686909842e-060.00011240903872531
HIV vs. Mock in Activation0.05613937949718160.9329659976519160.999983755607037
HIV vs. Mock in Latency-0.03732076150816940.8636699724965440.999834320637052
IL7 vs. CD3-0.4263383236315570.2362191942064090.365595226848055
SAHA vs. CD31.387415082188930.0002932107449774570.00123323637365623
SAHA vs. IL71.840595678190898.63517035654127e-111.39964040811294e-08
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.523458 0.41522
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef requires 25496667

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04721 Synaptic vesicle cycle - Homo sapiens (human)
hsa04961 Endocrine and other factor-regulated calcium reabsorption - Homo sapiens (human)
hsa05016 Huntington's disease - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)