Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0011280
UniProt IDO60266
Primary gene name(s)ADCY3
Synonym gene name(s)KIAA0511
Protein nameAdenylate cyclase type 3
Protein functionCatalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Participates in signaling cascades triggered by odorant receptors via its function in cAMP biosynthesis. Required for the perception of odorants. Required for normal sperm motility and normal male fertility. Plays a role in regulating insulin levels and body fat accumulation in response to a high fat diet. {ECO:0000250|UniProtKB:Q8VHH7}.
Subcellular locationCell membrane {ECO:0000269|PubMed:11549699};
Multi-pass membrane protein {ECO:0000305}. Cytoplasm {ECO:0000269|PubMed:11549699}. Cell projection, cilium {ECO:0000250|UniProtKB:Q8VHH7}. Golgi apparatus {ECO:0000250|UniProtKB:P21932}. Note=Also detected in the cytoplasm, close to lipid droplets. {ECO:0000269|PubMed:11549699}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60266
Gene Ontology
(Biological Process)
Complete annatation
acrosome reaction [GO:0007340];
activation of adenylate cyclase activity [GO:0007190];
activation of protein kinase A activity [GO:0034199];
adenylate cyclase-activating G-protein coupled receptor signaling pathway [GO:0007189];
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [GO:0007193];
cAMP biosynthetic process [GO:0006171];
cAMP-mediated signaling [GO:0019933];
cellular response to forskolin [GO:1904322];
cellular response to glucagon stimulus [GO:0071377];
flagellated sperm motility [GO:0030317];
olfactory learning [GO:0008355];
renal water homeostasis [GO:0003091];
sensory perception of smell [GO:0007608];
signal transduction [GO:0007165];
single fertilization [GO:0007338]
Gene Ontology
(Molecular Function)
Complete annatation
adenylate cyclase activity [GO:0004016];
ATP binding [GO:0005524];
calcium- and calmodulin-responsive adenylate cyclase activity [GO:0008294];
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
cilium [GO:0005929];
cytoplasm [GO:0005737];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
membrane raft [GO:0045121];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction106623
Phylogenetic treeO60266
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.488234985057739.21157145772344e-065.7418033503229e-05
AZA vs. DISU-0.05499807158022860.8288759899387830.985239814541943
AZA vs. IL70.3357250815158840.08345962650257850.794424696543271
AZA vs. SAHA0.5093013823546830.03918698389130510.233664792396176
DISU vs. CD3-1.556265893462842.66614129142972e-050.000176424116887672
DISU vs. IL70.3820360299810450.1306103183812150.473419223274062
DISU vs. SAHA0.5663962528638660.05368042736813420.290491102644578
DMSO vs. AZA0.08352065030707750.7133414774537581
DMSO vs. CD3-1.420742596861631.35651033682516e-057.59131806250779e-05
DMSO vs. DISU0.1355069401457320.5803016035112370.932784987235767
DMSO vs. IL70.2600876774801060.1508577413811290.652021626277804
DMSO vs. SAHA0.4207992059961090.07541108579919150.321204658498136
HIV vs. Mock in Activation0.122929211445630.8432520206507550.999983755607037
HIV vs. Mock in Latency-0.1050544104906650.5294952608260550.999834320637052
IL7 vs. CD3-1.145001912620360.0004280737066981730.00204641769142454
SAHA vs. CD3-1.003447961856890.005080101905937990.0144693131208629
SAHA vs. IL70.1700226540695180.4873631538710570.718526770757431
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.191504 0.674057
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 stimulates 10808179
Tat inhibits 11299302
Nef activates 11884542
Envelope transmembrane glycoprotein gp41 activates 10089566

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04211 Longevity regulating pathway - Homo sapiens (human)
hsa04213 Longevity regulating pathway - multiple species - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04270 Vascular smooth muscle contraction - Homo sapiens (human)
hsa04540 Gap junction - Homo sapiens (human)
hsa04611 Platelet activation - Homo sapiens (human)
hsa04713 Circadian entrainment - Homo sapiens (human)
hsa04723 Retrograde endocannabinoid signaling - Homo sapiens (human)
hsa04724 Glutamatergic synapse - Homo sapiens (human)
hsa04725 Cholinergic synapse - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)
hsa04740 Olfactory transduction - Homo sapiens (human)
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
hsa04911 Insulin secretion - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)
hsa04913 Ovarian steroidogenesis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa04918 Thyroid hormone synthesis - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04923 Regulation of lipolysis in adipocytes - Homo sapiens (human)
hsa04925 Aldosterone synthesis and secretion - Homo sapiens (human)
hsa04962 Vasopressin-regulated water reabsorption - Homo sapiens (human)
hsa04970 Salivary secretion - Homo sapiens (human)
hsa04971 Gastric acid secretion - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)
hsa04976 Bile secretion - Homo sapiens (human)
hsa05032 Morphine addiction - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05414 Dilated cardiomyopathy - Homo sapiens (human)