Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010960
UniProt IDO60331
Primary gene name(s)PIP5K1C
Synonym gene name(s)KIAA0589
Protein namePhosphatidylinositol 4-phosphate 5-kinase type-1 gamma
Protein functionCatalyzes the phosphorylation of phosphatidylinositol 4-phosphate, PtdIns4P to form phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5P2. PtdIns(4,5P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4,5P3, another second messenger. The majority of PtdIns(4,5P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2, adaptor protein complex 2. Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins, TLN1 and TLN2 to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins, TLN1 and TLN2 and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2, LFA-1 polarization and adhesion induced by T-cell receptor. Together with PIP5K1A have a role during embryogenesis and together with PIP5K1B may have a role immediately after birth. {ECO:0000269|PubMed:12422219, ECO:0000269|PubMed:12847086, ECO:0000269|PubMed:17261850, ECO:0000269|PubMed:17635937}.
Subcellular locationCell membrane;
Peripheral membrane protein;
Cytoplasmic side. Endomembrane system. Cytoplasm. Cell junction, focal adhesion. Cell junction, adherens junction. Cell projection, ruffle membrane. Cell projection, phagocytic cup. Cell projection, uropodium. Note=Detected in plasma membrane invaginations. Isoform 3 is detected in intracellular vesicle-like structures.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60331
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton organization [GO:0030036];
adherens junction assembly [GO:0034333];
clathrin-dependent endocytosis [GO:0072583];
neutrophil chemotaxis [GO:0030593];
phagocytosis [GO:0006909];
phosphatidylinositol biosynthetic process [GO:0006661];
regulation of phosphatidylinositol 3-kinase signaling [GO:0014066];
single organismal cell-cell adhesion [GO:0016337];
synaptic vesicle endocytosis [GO:0048488];
synaptic vesicle exocytosis [GO:0016079]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol-3-phosphate 4-kinase activity [GO:0052811];
1-phosphatidylinositol-4-phosphate 5-kinase activity [GO:0016308];
ATP binding [GO:0005524];
phosphatidylinositol-3,4-bisphosphate 5-kinase activity [GO:0052812]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endomembrane system [GO:0012505];
focal adhesion [GO:0005925];
nucleus [GO:0005634];
phagocytic cup [GO:0001891];
presynapse [GO:0098793];
ruffle membrane [GO:0032587];
synapse [GO:0045202];
uropod [GO:0001931]
Protein-protein interaction116969
Phylogenetic treeO60331
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.01763442339948540.9572395345054420.971892966128236
AZA vs. DISU0.03463377836137330.8922545748987090.993708567567281
AZA vs. IL7-0.1917087795858680.3406107678001710.999311006273513
AZA vs. SAHA0.2162209802806740.42103867590870.77445379597836
DISU vs. CD30.004507300324662690.9902712218657590.993831451291908
DISU vs. IL7-0.2358365138381250.3527169975042240.733059885079905
DISU vs. SAHA0.184365282055070.5370737668655330.844469352157585
DMSO vs. AZA0.0554028487265790.7438113073751861
DMSO vs. CD30.02446493874090620.9394493356694470.957898724047415
DMSO vs. DISU0.01843610475788430.9402199210326650.992373876590717
DMSO vs. IL7-0.2391092955562780.1878808520274180.699129415564804
DMSO vs. SAHA0.1565051255996330.5106397099413960.819841383671757
HIV vs. Mock in Activation0.5555253665836070.3751407176868730.999983755607037
HIV vs. Mock in Latency0.02385265804468850.8864884225845270.999834320637052
IL7 vs. CD3-0.2047564295440420.5278756473007030.654706944157758
SAHA vs. CD30.1756308832130970.6288087388110780.721649759554059
SAHA vs. IL70.4055541149459450.1496503757991750.366087271145207
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.121713 0.440938
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2G35 NMR - B=646-653.
3H1Z X-ray 1.8Å P=639-653.
3H85 X-ray 2.6Å P=646-653.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)