Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010831
UniProt IDO94972
Primary gene name(s)TRIM37
Synonym gene name(s)KIAA0898, MUL, POB1
Protein nameE3 ubiquitin-protein ligase TRIM37
Protein functionE3 ubiquitin-protein ligase required to prevent centriole reduplication, PubMed:15885686, PubMed:23769972. Probably acts by ubiquitinating positive regulators of centriole reduplication, PubMed:23769972. Mediates monoubiquitination of 'Lys-119' of histone H2A, H2AK119Ub, a specific tag for epigenetic transcriptional repression: associates with some Polycomb group, PcG multiprotein PRC2-like complex and mediates repression of target genes, PubMed:25470042. Has anti-HIV activity, PubMed:24317724. {ECO:0000269|PubMed:15885686, ECO:0000269|PubMed:23769972, ECO:0000269|PubMed:24317724, ECO:0000269|PubMed:25470042}.
Subcellular locationCytoplasm, perinuclear region {ECO:0000269|PubMed:15885686}. Peroxisome {ECO:0000269|PubMed:11938494}. Note=Found in vesicles of the peroxisome. Aggregates as aggresomes, a perinuclear region where certain misfolded or aggregated proteins are sequestered for proteasomal degradation. {ECO:0000269|PubMed:15885686}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94972
Gene Ontology
(Biological Process)
Complete annatation
aggresome assembly [GO:0070842];
histone H2A-K119 monoubiquitination [GO:0036353];
histone H2A monoubiquitination [GO:0035518];
negative regulation of centriole replication [GO:0046600];
negative regulation of NF-kappaB transcription factor activity [GO:0032088];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
protein autoubiquitination [GO:0051865];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
ligase activity [GO:0016874];
protein homodimerization activity [GO:0042803];
tumor necrosis factor receptor binding [GO:0005164];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
aggresome [GO:0016235];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
perinuclear region of cytoplasm [GO:0048471];
peroxisome [GO:0005777]
Protein-protein interaction110678
Phylogenetic treeO94972
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4273945514492180.1939459754494450.298007313892754
AZA vs. DISU0.3504470582208590.1685745788046110.74908949851251
AZA vs. IL70.1775844917050260.3598587858683670.999311006273513
AZA vs. SAHA-0.1362598035974580.5787956414903720.860331614464339
DISU vs. CD3-0.08962301923759930.8047111690784170.866258384823828
DISU vs. IL7-0.1815264334002380.4729885846981390.809250132144415
DISU vs. SAHA-0.4858720688824640.09784395206531230.399512390847598
DMSO vs. AZA-0.03710299399847060.8269567896845091
DMSO vs. CD3-0.4762428442678070.1384769978264730.222713352434697
DMSO vs. DISU-0.3894594420944390.112847317632790.589057525710474
DMSO vs. IL70.2218547517868390.2216203205143640.729610716916178
DMSO vs. SAHA-0.1063427547390850.6538205763525920.8895663712957
HIV vs. Mock in Activation0.006709415644815780.9914056030477830.999983755607037
HIV vs. Mock in Latency-0.07277684380949540.6628188043899920.999834320637052
IL7 vs. CD3-0.2410945716663990.4540432916414360.587853851134802
SAHA vs. CD3-0.5887936455608240.09802652764141070.171177262057481
SAHA vs. IL7-0.3176381397538590.1949004053934150.42640474759076
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.215306 0.573155
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3LRQ X-ray 2.2Å A/B/C/D=1-90.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)