Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010655
UniProt IDQ14703
Primary gene name(s)MBTPS1
Synonym gene name(s)KIAA0091, S1P, SKI1
Protein nameMembrane-bound transcription factor site-1 protease
Protein functionSerine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins, SREBPs. Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu, SERBP-2, Arg-His-Leu-Leu, ATF6, Arg-Gly-Leu-Thr, BDNF and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. {ECO:0000269|PubMed:21719679}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:9990022};
Single-pass type I membrane protein {ECO:0000269|PubMed:9990022}. Golgi apparatus membrane {ECO:0000269|PubMed:9990022};
Single-pass type I membrane protein {ECO:0000269|PubMed:9990022}. Note=May sort to other organelles, including lysosomal and/or endosomal compartments.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14703
Gene Ontology
(Biological Process)
Complete annatation
ATF6-mediated unfolded protein response [GO:0036500];
cholesterol metabolic process [GO:0008203];
lysosome organization [GO:0007040];
membrane protein intracellular domain proteolysis [GO:0031293];
proteolysis [GO:0006508];
regulation of transcription factor import into nucleus [GO:0042990];
response to endoplasmic reticulum stress [GO:0034976]
Gene Ontology
(Molecular Function)
Complete annatation
serine-type endopeptidase activity [GO:0004252]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum lumen [GO:0005788];
endoplasmic reticulum membrane [GO:0005789];
Golgi membrane [GO:0000139];
Golgi stack [GO:0005795];
integral component of membrane [GO:0016021]
Protein-protein interaction114259
Phylogenetic treeQ14703
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1087818814436110.7404012929010630.819033268741639
AZA vs. DISU0.08355896704860860.7407829467946870.975075282918539
AZA vs. IL7-0.1056588822753580.5819938326498230.999311006273513
AZA vs. SAHA-0.109301140513460.6537128243938380.894689461663588
DISU vs. CD30.1795641388617780.6221048920425250.729718243144427
DISU vs. IL7-0.1984394385717680.4303102533596540.784529994969834
DISU vs. SAHA-0.190998300042040.5116248194984270.83125244228771
DMSO vs. AZA0.03242944455094240.8461729811686911
DMSO vs. CD30.1279138375861560.6900241485803820.771673505928547
DMSO vs. DISU-0.05345727036493340.8263067981428860.980747352324641
DMSO vs. IL7-0.1306036174005080.4668803495493770.877167483330138
DMSO vs. SAHA-0.1473982961521260.5313006895797270.829433487980565
HIV vs. Mock in Activation0.1694494059728880.7852643992958020.999983755607037
HIV vs. Mock in Latency-0.0214439109464480.8963504965856140.999834320637052
IL7 vs. CD30.009964509321745430.9753452029353030.986064074113375
SAHA vs. CD3-0.02473874996824060.944428455945190.962112947413913
SAHA vs. IL7-0.006848056246786640.9775323814331260.991743742210813
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.00172801 0.992876
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
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