Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010532
UniProt IDQ99732
Primary gene name(s)LITAF
Synonym gene name(s)PIG7, SIMPLE
Protein nameLipopolysaccharide-induced tumor necrosis factor-alpha factor
Protein functionProbable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha, TNF-alpha gene expression.
Subcellular locationLysosome membrane {ECO:0000269|PubMed:11274176};
Peripheral membrane protein {ECO:0000269|PubMed:11274176};
Cytoplasmic side {ECO:0000269|PubMed:11274176}. Note=Associated with membranes of lysosomes.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99732
Gene Ontology
(Biological Process)
Complete annatation
aging [GO:0007568];
apoptotic process [GO:0006915];
cellular response to lipopolysaccharide [GO:0071222];
negative regulation of NF-kappaB import into nucleus [GO:0042347];
positive regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043123];
regulation of cytokine production [GO:0001817];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
signal transducer activity [GO:0004871];
WW domain binding [GO:0050699]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
Golgi apparatus [GO:0005794];
intracellular membrane-bounded organelle [GO:0043231];
lysosomal membrane [GO:0005765];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886]
Protein-protein interaction114893
Phylogenetic treeQ99732
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.208876177195660.0002677795339991460.00114658327739634
AZA vs. DISU-0.4302023549328430.08945788812382350.620097762154059
AZA vs. IL7-0.4251686312067380.02762763707254310.532956734665147
AZA vs. SAHA-0.1532811847595980.52962300231450.837726876903334
DISU vs. CD3-1.650575131559249.03510110128458e-066.83710411650421e-05
DISU vs. IL7-0.003958069967412690.9874696176510060.998505215881751
DISU vs. SAHA0.2775815643549920.3411767580860060.719645271052475
DMSO vs. AZA-0.03148018386202560.8508817038915331
DMSO vs. CD3-1.255377702673490.0001085660515729450.000484606686502641
DMSO vs. DISU0.3957927166658660.1051340519889580.578339386215277
DMSO vs. IL7-0.3859883913219620.03928311645944520.405611630565846
DMSO vs. SAHA-0.1276734612088320.5879895089450380.858791919403166
HIV vs. Mock in Activation-0.2432508010110480.6958408469779360.999983755607037
HIV vs. Mock in Latency0.05484835120824440.7398128734281530.999834320637052
IL7 vs. CD3-1.625302084615378.71668989321783e-078.63817664434401e-06
SAHA vs. CD3-1.386853565408370.0001132624397107710.000537425996990338
SAHA vs. IL70.2681032082568320.2729575286335670.520307217614459
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.44574 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif upregulates 23333304

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)