Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010522
UniProt IDO60291
Primary gene name(s)MGRN1
Synonym gene name(s)KIAA0544, RNF156
Protein nameE3 ubiquitin-protein ligase MGRN1
Protein functionE3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. {ECO:0000269|PubMed:17229889, ECO:0000269|PubMed:19703557, ECO:0000269|PubMed:19737927}.
Subcellular locationEarly endosome {ECO:0000269|PubMed:17229889, ECO:0000269|PubMed:19737927}. Note=The endosomal localization is dependent on the interaction with TSG101.;
SUBCELLULAR LOCATION: Isoform 1: Cytoplasm, cytosol. Nucleus. Note=Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, cytosol. Nucleus. Note=Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.;
SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, cytosol. Cell membrane.;
SUBCELLULAR LOCATION: Isoform 4: Cytoplasm, cytosol. Cell membrane.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60291
Gene Ontology
(Biological Process)
Complete annatation
endosome to lysosome transport [GO:0008333];
negative regulation of cAMP-mediated signaling [GO:0043951];
negative regulation of G-protein coupled receptor protein signaling pathway [GO:0045744];
protein monoubiquitination [GO:0006513];
protein polyubiquitination [GO:0000209]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome [GO:0005769];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction116890
Phylogenetic treeO60291
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.05277558448928990.8718817053151760.915366471336747
AZA vs. DISU0.133959537557610.5965209426233950.955158276076142
AZA vs. IL7-0.0593632492701760.777404488391540.999311006273513
AZA vs. SAHA-0.1082435009572240.6575399090619330.896891603873061
DISU vs. CD30.06899845718518530.8490612348318110.898102160869361
DISU vs. IL7-0.2030588182939670.4204250064838520.777963902873896
DISU vs. SAHA-0.2391742189386410.4126143477244970.768571355154603
DMSO vs. AZA0.05937500318248020.7235609556110381
DMSO vs. CD3-0.005395290215571030.9865415402159670.991146935868859
DMSO vs. DISU-0.07661979579295740.7534177944428720.969857060964769
DMSO vs. IL7-0.1110729144107930.5374100349902420.896696737481162
DMSO vs. SAHA-0.1721574919842430.4661965187127710.792443844769281
HIV vs. Mock in Activation0.415398416390820.5050920044688310.999983755607037
HIV vs. Mock in Latency0.03683502696456160.8238962442061040.999834320637052
IL7 vs. CD3-0.1075277785789950.7385540306312030.824487105888322
SAHA vs. CD3-0.1836157904778590.6030478981722390.700364659791587
SAHA vs. IL7-0.05105543842868410.8347091437873090.928453780508405
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0998018 0.539527
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)