Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010469
UniProt IDO75154
Primary gene name(s)RAB11FIP3
Synonym gene name(s)ARFO1, KIAA0665
Protein nameRab11 family-interacting protein 3
Protein functionActs as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. Also required for the structural integrity of the endosomal recycling compartment during interphase. May play a role in breast cancer cell motility by regulating actin cytoskeleton. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity, the ability to move along a microtubule for a long distance without falling off the track, PubMed:25035494. {ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:17394487, ECO:0000269|PubMed:19327867, ECO:0000269|PubMed:25035494}.
Subcellular locationRecycling endosome membrane {ECO:0000269|PubMed:11495908, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:17007872, ECO:0000269|PubMed:17394487};
Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:15158446, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:17394487, ECO:0000269|PubMed:18511905}. Cleavage furrow {ECO:0000269|PubMed:15158446, ECO:0000269|PubMed:15601896}. Midbody {ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:16148947, ECO:0000269|PubMed:18511905}. Note=In early mitosis remains diffuse and distributed through the cell. The onset of anaphase sequesters these vesicles to the centrosomes at the opposite poles of the cell. During telophase these vesicles move from the centrosomes, to the furrow, and then to the midbody to aid in abscission, PubMed:15158446, PubMed:15601896, PubMed:18511905. Interaction with Rab11 mediates localization to endosomes, PubMed:11495908. Interaction with ARF6 mediates localization to the midbody, PubMed:16148947. {ECO:0000269|PubMed:11495908, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:16148947, ECO:0000269|PubMed:18511905}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75154
Gene Ontology
(Biological Process)
Complete annatation
cytokinesis [GO:0000910];
endocytic recycling [GO:0032456];
negative regulation of adiponectin secretion [GO:0070164];
protein localization to cilium [GO:0061512];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
ADP-ribosylation factor binding [GO:0030306];
calcium ion binding [GO:0005509];
dynein light intermediate chain binding [GO:0051959];
protein homodimerization activity [GO:0042803];
Rab GTPase binding [GO:0017137]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cleavage furrow [GO:0032154];
cytosol [GO:0005829];
endosome [GO:0005768];
intercellular bridge [GO:0045171];
intracellular membrane-bounded organelle [GO:0043231];
microtubule organizing center [GO:0005815];
midbody [GO:0030496];
nucleoplasm [GO:0005654];
recycling endosome [GO:0055037];
recycling endosome membrane [GO:0055038]
Protein-protein interaction115076
Phylogenetic treeO75154
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.07344507137799790.8226811862785940.879268957565662
AZA vs. DISU0.1108509562329480.6646567472785320.961786723745098
AZA vs. IL7-0.13940672765140.4711788092652040.999311006273513
AZA vs. SAHA-0.1195219337669870.6254229192905960.880263780148127
DISU vs. CD30.02512194484289640.945407893476050.964363419855072
DISU vs. IL7-0.2594656081791740.3084955988491360.692113911201217
DISU vs. SAHA-0.2283181880904820.4387892929632890.784641803403784
DMSO vs. AZA0.04991488121863950.767567900030981
DMSO vs. CD3-0.03630645545455640.9097269117741150.938025391706592
DMSO vs. DISU-0.0630772990829850.7976539710855940.975622542347204
DMSO vs. IL7-0.1816182909338970.3156846397152350.796743000788074
DMSO vs. SAHA-0.1748670046948330.4599844230676870.788851301781192
HIV vs. Mock in Activation0.1215411818069120.8454105773825040.999983755607037
HIV vs. Mock in Latency-0.0599299544468860.7185850339874310.999834320637052
IL7 vs. CD3-0.2067612100190410.5204290962901160.647978503913837
SAHA vs. CD3-0.2169909866639520.5394060962300440.644788599205739
SAHA vs. IL70.01647984406190630.9462310645143850.978694161311944
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0473398 0.814351
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2D7C X-ray 1.7Å C/D=715-756.
2HV8 X-ray 1.8Å D/E/F=695-756.
4D0M X-ray 6.0Å E/F/K/L/U/V/a/b/e/f/i/j=713-756.
4UJ3 X-ray 3.0Å C/F/I/L/O/R/U/X=695-756.
4UJ4 X-ray 4.2Å C/F/I/L=695-756.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)