Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010373
UniProt IDQ9H223
Primary gene name(s)EHD4
Synonym gene name(s)HCA10, HCA11, PAST4
Protein nameEH domain-containing protein 4
Protein functionATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport. {ECO:0000269|PubMed:17233914, ECO:0000269|PubMed:18331452}.
Subcellular locationEarly endosome membrane {ECO:0000269|PubMed:18331452};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Recycling endosome membrane {ECO:0000269|PubMed:18331452};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Cell membrane {ECO:0000250|UniProtKB:Q9EQP2};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H223
Gene Ontology
(Biological Process)
Complete annatation
cellular response to growth factor stimulus [GO:0071363];
endocytic recycling [GO:0032456];
pinocytosis [GO:0006907];
positive regulation of peptidyl-tyrosine phosphorylation [GO:0050731];
protein homooligomerization [GO:0051260];
regulation of endocytosis [GO:0030100]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium ion binding [GO:0005509];
GTP binding [GO:0005525];
nucleic acid binding [GO:0003676]
Gene Ontology
(Cellular Component)
Complete annatation
early endosome membrane [GO:0031901];
endoplasmic reticulum [GO:0005783];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
recycling endosome membrane [GO:0055038]
Protein-protein interaction119054
Phylogenetic treeQ9H223
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7011844028979250.03382818434877110.0720037275108993
AZA vs. DISU-0.2929327549101630.2504769737366810.831594124314855
AZA vs. IL70.06623605421443010.7333738076846910.999311006273513
AZA vs. SAHA0.4322807034253280.07966708554235890.35124353538038
DISU vs. CD3-1.005806437930750.006095776489237980.0191704300374546
DISU vs. IL70.3497049727021770.1680296631186930.530565792706611
DISU vs. SAHA0.7273433307571220.01346276771074940.126179957816358
DMSO vs. AZA0.03788632817765450.8238559206872971
DMSO vs. CD3-0.6766551723424610.03602837126390820.0729428051459182
DMSO vs. DISU0.3284159117630580.1817289518719970.696088968126849
DMSO vs. IL70.03601147763393840.8430737954563970.965565824335307
DMSO vs. SAHA0.3893479228717480.1023859408485360.378200616305951
HIV vs. Mock in Activation0.04042126577050790.948266113842850.999983755607037
HIV vs. Mock in Latency-0.2888066768084810.0864938974780280.999834320637052
IL7 vs. CD3-0.6284887502615150.05208605695905180.11361695174295
SAHA vs. CD3-0.2930141026757810.4074872222908080.523349047790004
SAHA vs. IL70.3633729130498020.1391126147028770.350900862455015
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0686213 0.692239
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef cooperates with 23325686

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)