Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0010136
UniProt IDQ8N3Y1
Primary gene name(s)FBXW8
Synonym gene name(s)FBW6, FBW8, FBX29, FBXO29, FBXW6
Protein nameF-box/WD repeat-containing protein 8
Protein functionSubstrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8 complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain, PubMed:21572988. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8 complex recognizes and binds IRS1 previously phosphorylated by S6 kinase, RPS6KB1 or RPS6KB2, PubMed:18498745. The Cul7-RING(FBXW8 complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradatation, thereby affecting cell proliferation and differentiation, PubMed:24362026. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions, PubMed:24793695. {ECO:0000269|PubMed:18498745, ECO:0000269|PubMed:21572988, ECO:0000269|PubMed:24362026, ECO:0000269|PubMed:24793695}.
Subcellular locationCytoplasm, perinuclear region {ECO:0000269|PubMed:21572988}. Golgi apparatus {ECO:0000269|PubMed:21572988}. Note=Colocalizes with CUL7 at the Golgi apparatus in neurons. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N3Y1
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
Golgi organization [GO:0007030];
labyrinthine layer blood vessel development [GO:0060716];
positive regulation of dendrite morphogenesis [GO:0050775];
protein ubiquitination [GO:0016567];
spongiotrophoblast layer development [GO:0060712]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
Cul7-RING ubiquitin ligase complex [GO:0031467];
cytoplasm [GO:0005737];
Golgi apparatus [GO:0005794];
perinuclear region of cytoplasm [GO:0048471];
SCF ubiquitin ligase complex [GO:0019005]
Protein-protein interaction117645
Phylogenetic treeQ8N3Y1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.460425612165721.58554013985901e-059.33851543101016e-05
AZA vs. DISU-0.4162921721378210.1054188587808970.653579050610225
AZA vs. IL70.1201188811865280.5413547352986680.999311006273513
AZA vs. SAHA0.355924038147890.1501982701234810.494018922941599
DISU vs. CD31.032373892725740.005367337938021130.017185057790512
DISU vs. IL70.5268509868018250.03950945094069250.261804383705907
DISU vs. SAHA0.7744957472037090.008807597058316240.094813966855248
DMSO vs. AZA0.05061844012784050.769815578244221
DMSO vs. CD31.500658836363675.793322194525e-063.52631914587956e-05
DMSO vs. DISU0.4652122624518660.06058935815293420.474720361980936
DMSO vs. IL70.07690925490207160.6760075271152230.933481127714756
DMSO vs. SAHA0.2995503041719740.2097714656920270.551354335445508
HIV vs. Mock in Activation-0.08795142947397510.8897662223305810.999983755607037
HIV vs. Mock in Latency0.1008452982438770.5513303476163960.999834320637052
IL7 vs. CD31.586513313880211.86835704130139e-061.71196022392316e-05
SAHA vs. CD31.793008290034041.04598039718518e-068.43388153321594e-06
SAHA vs. IL70.2339414675151180.3422622235953530.591658343861966
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0099438 0.974687
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
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