Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009882
UniProt IDQ92993
Primary gene name(s)KAT5
Synonym gene name(s)HTATIP, TIP60
Protein nameHistone acetyltransferase KAT5
Protein functionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Promotes FOXP3 acetylation and positively regulates its transcriptional repressor activity, PubMed:17360565. {ECO:0000269|PubMed:12776177, ECO:0000269|PubMed:14966270, ECO:0000269|PubMed:15042092, ECO:0000269|PubMed:15121871, ECO:0000269|PubMed:15310756, ECO:0000269|PubMed:16141325, ECO:0000269|PubMed:16387653, ECO:0000269|PubMed:17360565, ECO:0000269|PubMed:17996965, ECO:0000269|PubMed:19909775, ECO:0000269|PubMed:24463511}.
Subcellular locationNucleus {ECO:0000269|PubMed:17360565}. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q92993
Gene Ontology
(Biological Process)
Complete annatation
androgen receptor signaling pathway [GO:0030521];
beta-catenin-TCF complex assembly [GO:1904837];
cellular response to estradiol stimulus [GO:0071392];
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [GO:0006978];
DNA double-strand break processing [GO:0000729];
DNA replication [GO:0006260];
DNA synthesis involved in DNA repair [GO:0000731];
double-strand break repair [GO:0006302];
double-strand break repair via nonhomologous end joining [GO:0006303];
histone acetylation [GO:0016573];
negative regulation of interleukin-2 production [GO:0032703];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of protein acetylation [GO:1901985];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
regulation of growth [GO:0040008];
regulation of signal transduction by p53 class mediator [GO:1901796];
response to ionizing radiation [GO:0010212];
strand displacement [GO:0000732];
transcription, DNA-templated [GO:0006351];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
acetyltransferase activity [GO:0016407];
androgen receptor binding [GO:0050681];
histone acetyltransferase activity [GO:0004402];
metal ion binding [GO:0046872];
repressing transcription factor binding [GO:0070491];
transcription coactivator activity [GO:0003713]
Gene Ontology
(Cellular Component)
Complete annatation
NuA4 histone acetyltransferase complex [GO:0035267];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
Piccolo NuA4 histone acetyltransferase complex [GO:0032777];
Swr1 complex [GO:0000812];
transcription factor complex [GO:0005667]
Protein-protein interaction115779
Phylogenetic treeQ92993
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.295948375951810.3681342492506090.489978753506286
AZA vs. DISU0.5381797478770.03439481783454670.435307126959848
AZA vs. IL7-0.02649694861349710.8912958107346340.999311006273513
AZA vs. SAHA-0.9769715281135310.006342630031817010.0722653289516069
DISU vs. CD30.2289579888646940.5281647877426880.64986988902402
DISU vs. IL7-0.5743881189743120.02344018925570190.194435561407393
DISU vs. SAHA-1.512439039087572.42017126030891e-050.00129802139018205
DMSO vs. AZA0.06992661963061260.6798162045331891
DMSO vs. CD3-0.2378749597147240.4587534776382910.569942366866308
DMSO vs. DISU-0.4702688334603190.05510689076602460.457565398072315
DMSO vs. IL7-0.08902668282302560.6234522656319190.91811145037322
DMSO vs. SAHA-1.052090059423420.002716476439223130.036166092239869
HIV vs. Mock in Activation0.1350979816196390.8283025369214330.999983755607037
HIV vs. Mock in Latency0.1109925458342320.5057298229804150.999834320637052
IL7 vs. CD3-0.3169846573650110.3263951015498650.464609818270008
SAHA vs. CD3-1.296019777278470.0006002740251501710.00229480709259591
SAHA vs. IL7-0.9531101872039810.006942785334635550.0485415334574658
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.202605 0.654553
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01992 Coenzyme A nutraceutical unknown unknown
DB02039 S-Acetyl-Cysteine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2EKO NMR - A=5-78.
2OU2 X-ray 2.3Å A=227-506.
4QQG X-ray 2.8Å A/B/C/D/E/F/G=1-80.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat inhibits 10393559
Tat enhanced by 8607265
Tat complexes with 22391203
Tat inhibited by 11275565
Tat degrades 16001085
Tat binds 8607265
Tat downregulates 21320689
matrix downregulates 26469385

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05166 HTLV-I infection - Homo sapiens (human)