Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009609
UniProt IDP17655
Primary gene name(s)CAPN2
Synonym gene name(s)CANPL2
Protein nameCalpain-2 catalytic subunit
Protein functionCalcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226', PubMed:17650508. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs, By similarity. {ECO:0000250|UniProtKB:O08529, ECO:0000269|PubMed:17650508}.
Subcellular locationCytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+ binding.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P17655
Gene Ontology
(Biological Process)
Complete annatation
blastocyst development [GO:0001824];
cellular response to amino acid stimulus [GO:0071230];
extracellular matrix disassembly [GO:0022617];
myoblast fusion [GO:0007520];
protein autoprocessing [GO:0016540];
proteolysis [GO:0006508];
proteolysis involved in cellular protein catabolic process [GO:0051603];
regulation of cytoskeleton organization [GO:0051493];
response to hypoxia [GO:0001666]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-dependent cysteine-type endopeptidase activity [GO:0004198];
calcium ion binding [GO:0005509];
cysteine-type peptidase activity [GO:0008234];
cytoskeletal protein binding [GO:0008092]
Gene Ontology
(Cellular Component)
Complete annatation
chromatin [GO:0000785];
cortical actin cytoskeleton [GO:0030864];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dendrite [GO:0030425];
endoplasmic reticulum [GO:0005783];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
Golgi apparatus [GO:0005794];
lysosome [GO:0005764];
membrane raft [GO:0045121];
nucleus [GO:0005634];
perinuclear endoplasmic reticulum [GO:0097038];
plasma membrane [GO:0005886];
pseudopodium [GO:0031143]
Protein-protein interaction107274
Phylogenetic treeP17655
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.702972720732874.85738709699035e-074.0358620907298e-06
AZA vs. DISU-0.08738738423188070.7291930797274850.974560663114398
AZA vs. IL70.03354026568764580.8610763893684320.999311006273513
AZA vs. SAHA-0.9424866337143490.0003077438411924760.00904669357409982
DISU vs. CD31.602331995031161.92575012183394e-050.000132611956768044
DISU vs. IL70.1119673968419310.656615058127530.901812730840794
DISU vs. SAHA-0.8535089893540730.004448981785141190.0594947688410496
DMSO vs. AZA0.02907200005927660.8615978999818571
DMSO vs. CD31.718481374866981.938482492303e-071.60369240782782e-06
DMSO vs. DISU0.1141189038540230.6394595691751270.9472626202031
DMSO vs. IL70.01195590687922930.9467917112997040.990279530960645
DMSO vs. SAHA-0.9775064045063190.00012546686746040.00415584870218832
HIV vs. Mock in Activation0.3009591250652440.6289113163342860.999983755607037
HIV vs. Mock in Latency0.02241585348457120.8914483136938980.999834320637052
IL7 vs. CD31.743936085336491.38867589871694e-071.64168720167533e-06
SAHA vs. CD30.736012098197310.03823068676217870.0795219392002805
SAHA vs. IL7-0.9795823341346567.79462267361808e-050.00159641497530053
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0159167 0.940492
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1KFU X-ray 2.5Å L=2-700.
1KFX X-ray 3.1Å L=2-700.
2NQA X-ray 2.2Å A/B=48-346.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 20962236
Envelope surface glycoprotein gp120 activates 8954122

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa05010 Alzheimer's disease - Homo sapiens (human)