Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009511
UniProt IDQ99755
Primary gene name(s)PIP5K1A
Synonym gene name(s)unknown
Protein namePhosphatidylinositol 4-phosphate 5-kinase type-1 alpha
Protein functionCatalyzes the phosphorylation of phosphatidylinositol 4-phosphate, PtdIns4P to form phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5P2. PtdIns(4,5P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4,5P3, another second messenger. The majority of PtdIns(4,5P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5P2 for the production of PtdIns(3,4,5P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. {ECO:0000269|PubMed:18288197, ECO:0000269|PubMed:19158393, ECO:0000269|PubMed:20660631}.
Subcellular locationCell membrane. Cytoplasm {ECO:0000250}. Nucleus speckle. Cell projection, ruffle. Note=Colocalizes with RAC1 at actin-rich membrane ruffles. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99755
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton reorganization [GO:0031532];
activation of GTPase activity [GO:0090630];
cell chemotaxis [GO:0060326];
cell migration [GO:0016477];
fibroblast migration [GO:0010761];
focal adhesion assembly [GO:0048041];
glycerophospholipid metabolic process [GO:0006650];
keratinocyte differentiation [GO:0030216];
phagocytosis [GO:0006909];
phosphatidylinositol biosynthetic process [GO:0006661];
phospholipid biosynthetic process [GO:0008654];
protein targeting to plasma membrane [GO:0072661];
regulation of phosphatidylinositol 3-kinase signaling [GO:0014066];
ruffle assembly [GO:0097178];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol-3-phosphate 4-kinase activity [GO:0052811];
1-phosphatidylinositol-3-phosphate 5-kinase activity [GO:0000285];
1-phosphatidylinositol-4-phosphate 5-kinase activity [GO:0016308];
1-phosphatidylinositol-5-kinase activity [GO:0052810];
ATP binding [GO:0005524];
kinase binding [GO:0019900];
phosphatidylinositol-3,4-bisphosphate 5-kinase activity [GO:0052812]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
focal adhesion [GO:0005925];
lamellipodium [GO:0030027];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
ruffle membrane [GO:0032587]
Protein-protein interaction113983
Phylogenetic treeQ99755
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9830008734170880.002926988945319620.00922534031448292
AZA vs. DISU0.1290145662235680.6105928786519550.957450739791279
AZA vs. IL70.1099420720243130.5684655981876560.999311006273513
AZA vs. SAHA0.1346832056328120.5816714363926020.861054230794665
DISU vs. CD3-0.8667205635866950.01774174998709440.0465435327715735
DISU vs. IL7-0.02813277364786250.9111815004268130.982189124739072
DISU vs. SAHA0.007269456858359880.9801564772518060.996555236366591
DMSO vs. AZA-0.04453542776728970.7912714570082381
DMSO vs. CD3-1.039686053912110.001287899137198730.00426234633170011
DMSO vs. DISU-0.1756086100048560.4723983131997740.897275884208221
DMSO vs. IL70.1618631045770840.3694734189889470.828851593822924
DMSO vs. SAHA0.1729433460474170.4642224331395890.792317765830935
HIV vs. Mock in Activation0.08316798899177970.8935745497592690.999983755607037
HIV vs. Mock in Latency-0.06809079422286570.6804396861439650.999834320637052
IL7 vs. CD3-0.8657801125997660.00743922023947530.0234192495132855
SAHA vs. CD3-0.8729697267915170.01407595673874610.0342081386958399
SAHA vs. IL70.02176524014009620.9289003411787640.973662731585334
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.175421 0.23302
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 activates 18981107

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)