Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009494
UniProt IDQ9Y6X2
Primary gene name(s)PIAS3
Synonym gene name(s)unknown
Protein nameE3 SUMO-protein ligase PIAS3
Protein functionFunctions as an E3-type small ubiquitin-like modifier, SUMO ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation, PubMed:21965678, PubMed:9388184. Sumoylates CCAR2 which promotes its interaction with SIRT1, PubMed:25406032. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus, PubMed:24651376. {ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:24651376, ECO:0000269|PubMed:25406032, ECO:0000269|PubMed:9388184}.
Subcellular locationCytoplasm {ECO:0000250|UniProtKB:O54714}. Nucleus {ECO:0000250|UniProtKB:O54714}. Nucleus speckle {ECO:0000250|UniProtKB:O54714}. Note=Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules. {ECO:0000250|UniProtKB:O54714}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y6X2
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of protein sumoylation [GO:0033234];
positive regulation of gene expression [GO:0010628];
positive regulation of membrane potential [GO:0045838];
positive regulation of protein sumoylation [GO:0033235];
protein sumoylation [GO:0016925];
regulation of transcription, DNA-templated [GO:0006355];
response to hormone [GO:0009725];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
ligase activity [GO:0016874];
potassium channel regulator activity [GO:0015459];
protein C-terminus binding [GO:0008022];
SUMO transferase activity [GO:0019789];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
dendrite [GO:0030425];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
synapse [GO:0045202]
Protein-protein interaction115673
Phylogenetic treeQ9Y6X2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9917115016563070.002860540439920120.00903669367228591
AZA vs. DISU0.1999419610784590.4327827596763020.915650781529304
AZA vs. IL7-0.1480006534242530.4703526018760810.999311006273513
AZA vs. SAHA0.1737260742642790.4794663770526240.808696838080511
DISU vs. CD31.178526629873230.00137522224327480.00543384012978357
DISU vs. IL7-0.357902694582610.1588132645978660.516974727664182
DISU vs. SAHA-0.02302345437352160.9372969011017160.985272223282263
DMSO vs. AZA0.09329321659543830.5847386867976371
DMSO vs. CD31.074621662583760.0009499602852829360.0032711203880657
DMSO vs. DISU-0.1080952337209190.6600318754828130.95067670292921
DMSO vs. IL7-0.2340353035182370.1989987363455260.709700958223042
DMSO vs. SAHA0.07546482483308270.7503644988185290.927241300088349
HIV vs. Mock in Activation0.4557299896180690.4674097212873070.999983755607037
HIV vs. Mock in Latency0.1387544119102210.4067001173715870.999834320637052
IL7 vs. CD30.8477996856354580.009176253492490180.0277515387023083
SAHA vs. CD31.142312033302230.001473693107692740.00499405332424772
SAHA vs. IL70.3195823778013180.1926998240861510.423187230226492
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.128466 0.962382
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4MVT X-ray 2.3Å A/B/C/D=112-467.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 upregulates 25008924
26116514

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
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