Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009411
UniProt IDO95405
Primary gene name(s)ZFYVE9
Synonym gene name(s)MADHIP, SARA, SMADIP
Protein nameZinc finger FYVE domain-containing protein 9
Protein functionEarly endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. {ECO:0000269|PubMed:15356634, ECO:0000269|PubMed:9865696}.
Subcellular locationCytoplasm. Early endosome membrane.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95405
Gene Ontology
(Biological Process)
Complete annatation
endocytosis [GO:0006897];
SMAD protein complex assembly [GO:0007183];
SMAD protein import into nucleus [GO:0007184];
transforming growth factor beta receptor signaling pathway [GO:0007179]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol binding [GO:0005545];
metal ion binding [GO:0046872];
serine-type peptidase activity [GO:0008236]
Gene Ontology
(Cellular Component)
Complete annatation
early endosome [GO:0005769];
early endosome membrane [GO:0031901]
Protein-protein interaction114773
Phylogenetic treeO95405
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.112786545076480.02288209395066690.0520100919958454
AZA vs. DISU-0.04622680544036460.9268183151807040.995042856396371
AZA vs. IL7-0.06910908449727360.8575656302127770.999311006273513
AZA vs. SAHA-0.1379430450944620.8243446264259870.957305163458707
DISU vs. CD3-1.176496357548850.03763480565440560.0859622224741024
DISU vs. IL7-0.02545446027082380.9570964937825210.992802994773704
DISU vs. SAHA-0.0938503792025830.8879233747670860.971874193603508
DMSO vs. AZA-0.07633545533335320.852677068954681
DMSO vs. CD3-1.209986159649160.009267042282626980.0233575358855238
DMSO vs. DISU-0.033079837703160.9458264737502030.992938081932553
DMSO vs. IL70.01721260534301550.9623166625651830.993151606293804
DMSO vs. SAHA-0.07097634723877320.9070821879545550.977961819892905
HIV vs. Mock in Activation-1.139122726385910.08099284062930120.999983755607037
HIV vs. Mock in Latency-0.3867253678266040.3845261817757760.999834320637052
IL7 vs. CD3-1.173061783015630.009594122961508190.0288186957749727
SAHA vs. CD3-1.282805056187210.0628579789883050.119862045217482
SAHA vs. IL7-0.08250151806102470.8905367090855780.956735111735158
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.1452 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1DEV X-ray 2.2Å B/D=771-811.
1MK2 X-ray 2.7Å B=773-810.
4BKW X-ray 2.5Å A=895-1425.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef requires 25496667

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)