Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009311
UniProt IDQ9UI95
Primary gene name(s)MAD2L2
Synonym gene name(s)MAD2B, REV7
Protein nameMitotic spindle assembly checkpoint protein MAD2B
Protein functionAdapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. {ECO:0000269|PubMed:11459825, ECO:0000269|PubMed:11459826, ECO:0000269|PubMed:17296730, ECO:0000269|PubMed:17719540, ECO:0000269|PubMed:19443654}.
Subcellular locationNucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UI95
Gene Ontology
(Biological Process)
Complete annatation
actin filament organization [GO:0007015];
cell division [GO:0051301];
DNA damage response, signal transduction resulting in transcription [GO:0042772];
double-strand break repair [GO:0006302];
error-prone translesion synthesis [GO:0042276];
mitotic nuclear division [GO:0007067];
mitotic spindle assembly checkpoint [GO:0007094];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of cell-cell adhesion mediated by cadherin [GO:2000048];
negative regulation of epithelial to mesenchymal transition [GO:0010719];
negative regulation of protein catabolic process [GO:0042177];
negative regulation of sequence-specific DNA binding transcription factor activity [GO:0043433];
negative regulation of transcription by competitive promoter binding [GO:0010944];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
negative regulation of transcription regulatory region DNA binding [GO:2000678];
negative regulation of ubiquitin protein ligase activity [GO:1904667];
positive regulation of peptidyl-serine phosphorylation [GO:0033138];
positive regulation of transcription, DNA-templated [GO:0045893];
regulation of cell growth [GO:0001558];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
JUN kinase binding [GO:0008432];
RNA polymerase II activating transcription factor binding [GO:0001102]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
spindle [GO:0005819];
zeta DNA polymerase complex [GO:0016035]
Protein-protein interaction115722
Phylogenetic treeQ9UI95
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.841814018809660.01182842088295550.0301094341408387
AZA vs. DISU-0.1785364897002720.6540201104172940.960057478314726
AZA vs. IL70.4357184350606910.1317273660396780.897867143068752
AZA vs. SAHA0.5355220528092330.1568288416892910.50438189878907
DISU vs. CD3-1.030243606881940.009293235732325770.0272382336047207
DISU vs. IL70.6036160185635130.09335441886954690.406152341835042
DISU vs. SAHA0.7173567614957810.101212652937290.40655007793654
DMSO vs. AZA0.04004237897218930.8875391720246551
DMSO vs. CD3-0.8098366751139680.01285192383690970.030893199146796
DMSO vs. DISU0.2174841162278050.5435415506833230.922272808496102
DMSO vs. IL70.4026830871574990.07536391713464790.519571686094572
DMSO vs. SAHA0.4910699045597220.1376934212879970.444030915184205
HIV vs. Mock in Activation0.07855534078941910.9000837816091890.999983755607037
HIV vs. Mock in Latency0.0888559658673620.6566219150183870.999834320637052
IL7 vs. CD3-0.4022221248499430.2142329096907330.339729894557436
SAHA vs. CD3-0.327591747230920.391144507579670.507800459099382
SAHA vs. IL70.1003664708861770.7680631473696520.897154892169712
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.268167 0.0991221
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3ABD X-ray 1.9Å A/B=1-211.
3ABE X-ray 2.6Å C=1-211.
3VU7 X-ray 2.8Å C=1-211.
4EXT X-ray 1.9Å C=7-209.
4GK0 X-ray 2.7Å A/B=1-211.
4GK5 X-ray 3.2Å A/B=1-211.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05131 Shigellosis - Homo sapiens (human)