Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009129
UniProt IDQ9NRD1
Primary gene name(s)FBXO6
Synonym gene name(s)FBG2, FBS2, FBX6
Protein nameF-box only protein 6
Protein functionSubstrate-recognition component of some SCF, SKP1-CUL1-F-box protein-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway, ERAD for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1, phosphorylated on 'Ser-345', promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication. {ECO:0000269|PubMed:18203720, ECO:0000269|PubMed:19716789}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:19716789}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NRD1
Gene Ontology
(Biological Process)
Complete annatation
DNA damage checkpoint [GO:0000077];
DNA repair [GO:0006281];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
glycoprotein catabolic process [GO:0006516];
protein polyubiquitination [GO:0000209];
proteolysis [GO:0006508];
response to unfolded protein [GO:0006986];
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [GO:0031146]
Gene Ontology
(Molecular Function)
Complete annatation
carbohydrate binding [GO:0030246];
glycoprotein binding [GO:0001948];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum quality control compartment [GO:0044322];
SCF ubiquitin ligase complex [GO:0019005]
Protein-protein interaction117654
Phylogenetic treeQ9NRD1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3527268983451560.2906551192365640.408043401372344
AZA vs. DISU-0.4415156424698720.3101247985707050.866242136589333
AZA vs. IL70.7356603301801250.04135442144462590.616553110222399
AZA vs. SAHA-1.464808551754510.005675455694986060.0674698889165772
DISU vs. CD3-0.09827161203085050.7894347737985140.855078979733842
DISU vs. IL71.166287136882820.002866912434309370.0534296496894467
DISU vs. SAHA-1.019788857249280.05919316172937560.307130398280279
DMSO vs. AZA0.0180234375460880.957097896849341
DMSO vs. CD30.3628780419464660.2651450190112610.37252833305322
DMSO vs. DISU0.4585409794639240.2143317802172880.726625098239347
DMSO vs. IL70.724735324888610.00842131477177410.189830215695712
DMSO vs. SAHA-1.485324069377250.001651962552267410.0257197599434112
HIV vs. Mock in Activation0.1638579917942270.7951581615770240.999983755607037
HIV vs. Mock in Latency0.2362980075595540.4677782158303880.999834320637052
IL7 vs. CD31.091397620229930.0009358510595763870.00400513430465499
SAHA vs. CD3-1.13042709177340.00932319075305010.0241700472472845
SAHA vs. IL7-2.199901408574677.53760798588576e-060.000241748729317536
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.9 0.005732467 1.3 0.090730112 1.7 0.036417463
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.39887 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.088 5.92E-04

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope transmembrane glycoprotein gp41 interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)