Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0009064
UniProt IDP01112
Primary gene name(s)HRAS
Synonym gene name(s)HRAS1
Protein nameGTPase HRas
Protein functionRas proteins bind GDP/GTP and possess intrinsic GTPase activity. {ECO:0000269|PubMed:12740440, ECO:0000269|PubMed:14500341, ECO:0000269|PubMed:9020151}.
Subcellular locationCell membrane. Cell membrane;
Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane;
Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form, By similarity. Shuttles between the plasma membrane and the Golgi apparatus. {ECO:0000250}.;
SUBCELLULAR LOCATION: Isoform 2: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Colocalizes with RACK1 to the perinuclear region.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P01112
Gene Ontology
(Biological Process)
Complete annatation
animal organ morphogenesis [GO:0009887];
axon guidance [GO:0007411];
cell cycle arrest [GO:0007050];
cell proliferation [GO:0008283];
cell surface receptor signaling pathway [GO:0007166];
cellular senescence [GO:0090398];
chemotaxis [GO:0006935];
defense response to protozoan [GO:0042832];
endocytosis [GO:0006897];
ephrin receptor signaling pathway [GO:0048013];
epidermal growth factor receptor signaling pathway [GO:0007173];
ERBB2 signaling pathway [GO:0038128];
Fc-epsilon receptor signaling pathway [GO:0038095];
intrinsic apoptotic signaling pathway [GO:0097193];
leukocyte migration [GO:0050900];
MAPK cascade [GO:0000165];
mitotic cell cycle checkpoint [GO:0007093];
negative regulation of cell proliferation [GO:0008285];
negative regulation of gene expression [GO:0010629];
negative regulation of GTPase activity [GO:0034260];
negative regulation of neuron apoptotic process [GO:0043524];
positive regulation of actin cytoskeleton reorganization [GO:2000251];
positive regulation of cell migration [GO:0030335];
positive regulation of cell proliferation [GO:0008284];
positive regulation of DNA replication [GO:0045740];
positive regulation of epithelial cell proliferation [GO:0050679];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of GTPase activity [GO:0043547];
positive regulation of interferon-gamma production [GO:0032729];
positive regulation of JNK cascade [GO:0046330];
positive regulation of MAPK cascade [GO:0043410];
positive regulation of MAP kinase activity [GO:0043406];
positive regulation of miRNA metabolic process [GO:2000630];
positive regulation of protein phosphorylation [GO:0001934];
positive regulation of Ras protein signal transduction [GO:0046579];
positive regulation of ruffle assembly [GO:1900029];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of wound healing [GO:0090303];
protein heterooligomerization [GO:0051291];
Ras protein signal transduction [GO:0007265];
regulation of long-term neuronal synaptic plasticity [GO:0048169];
signal transduction [GO:0007165];
social behavior [GO:0035176];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
T cell receptor signaling pathway [GO:0050852];
T-helper 1 type immune response [GO:0042088]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
protein C-terminus binding [GO:0008022]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886]
Protein-protein interaction109501
Phylogenetic treeP01112
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9099711267963690.006146774977391250.0172618208420423
AZA vs. DISU0.0589356738328550.8180431318421030.984256604838454
AZA vs. IL70.166868599352840.3965889065550080.999311006273513
AZA vs. SAHA-0.0298196564972820.9043285122665130.978791439887516
DISU vs. CD3-0.8628566817609130.01856627439998710.048278078369054
DISU vs. IL70.09782872577406180.7008684668682790.923485022116887
DISU vs. SAHA-0.08607731970222080.7699716165737290.934921224088195
DMSO vs. AZA0.04851344671836260.7794435401583451
DMSO vs. CD3-0.8723471853322420.007061019424373430.0184453368524685
DMSO vs. DISU-0.01232902345813740.960180127343080.994105021699463
DMSO vs. IL70.1258552728998110.4942228737995060.885378142451182
DMSO vs. SAHA-0.08340504906817740.7277816702182310.919729758959119
HIV vs. Mock in Activation0.194009257553680.7565266302557210.999983755607037
HIV vs. Mock in Latency-0.04779556716039880.7795425527172170.999834320637052
IL7 vs. CD3-0.7382987778765620.0228765376414820.0582689671220898
SAHA vs. CD3-0.9627752605614240.007456408482236170.0200478279798928
SAHA vs. IL7-0.198268511456720.4228792887024970.66525593799848
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.231140227
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.461052 0.00108419
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02210 Hexane-1,6-Diol experimental unknown unknown
DB03226 Trifluoroethanol experimental unknown unknown
DB04137 Guanosine-5&,39;-Triphosphate experimental unknown unknown
DB04315 Guanosine-5&,39;-Diphosphate experimental unknown unknown
DB08751 N,N&,39;-DIMETHYL-N-(ACETYL)-N&,39;-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
121P X-ray 1.5Å A=1-166.
1AA9 NMR - A=1-171.
1AGP X-ray 2.3Å A=1-166.
1BKD X-ray 2.8Å R=1-166.
1CLU X-ray 1.7Å A=1-166.
1CRP NMR - A=1-166.
1CRQ NMR - A=1-166.
1CRR NMR - A=1-166.
1CTQ X-ray 1.2Å A=1-166.
1GNP X-ray 2.7Å A=1-166.
1GNQ X-ray 2.5Å A=1-166.
1GNR X-ray 1.8Å A=1-166.
1HE8 X-ray 3.0Å B=1-166.
1IAQ X-ray 2.9Å A/B/C=1-166.
1IOZ X-ray 2.0Å A=1-171.
1JAH X-ray 1.8Å A=1-166.
1JAI X-ray 1.8Å A=1-166.
1K8R X-ray 3.0Å A=1-166.
1LF0 X-ray 1.7Å A=1-166.
1LF5 X-ray 1.7Å A=1-166.
1LFD X-ray 2.1Å B/D=1-167.
1NVU X-ray 2.2Å Q/R=1-166.
1NVV X-ray 2.1Å Q/R=1-166.
1NVW X-ray 2.7Å Q/R=1-166.
1NVX X-ray 3.2Å Q/R=1-166.
1P2S X-ray 2.4Å A=1-166.
1P2T X-ray 2.0Å A=1-166.
1P2U X-ray 2.0Å A=1-166.
1P2V X-ray 2.3Å A=1-166.
1PLJ X-ray 2.8Å A=1-166.
1PLK X-ray 2.8Å A=1-166.
1PLL X-ray 2.8Å A=1-166.
1Q21 X-ray 2.2Å A=1-171.
1QRA X-ray 1.6Å A=1-166.
1RVD X-ray 1.9Å A=1-166.
1WQ1 X-ray 2.5Å R=1-166.
1XCM X-ray 1.8Å A=1-167.
1XD2 X-ray 2.7Å A/B=1-166.
1XJ0 X-ray 1.7Å A=1-166.
1ZVQ X-ray 2.0Å A=1-166.
1ZW6 X-ray 1.5Å A=1-166.
221P X-ray 2.3Å A=1-166.
2C5L X-ray 1.9Å A/B=1-166.
2CE2 X-ray 1.0Å X=1-166.
2CL0 X-ray 1.8Å X=1-166.
2CL6 X-ray 1.2Å X=1-166.
2CL7 X-ray 1.2Å X=1-166.
2CLC X-ray 1.3Å X=1-166.
2CLD X-ray 1.2Å X=1-166.
2EVW X-ray 1.0Å X=1-166.
2GDP Model - A=1-171.
2LCF NMR - A=1-166.
2LWI NMR - A=1-166.
2N42 NMR - A=1-166.
2N46 NMR - A=1-166.
2Q21 X-ray 2.2Å A=1-171.
2QUZ X-ray 1.4Å A=1-166.
2RGA X-ray 1.9Å A=1-166.
2RGB X-ray 1.3Å A=1-166.
2RGC X-ray 1.6Å A=1-166.
2RGD X-ray 2.0Å A=1-166.
2RGE X-ray 1.4Å A=1-166.
2RGG X-ray 1.4Å A=1-166.
2UZI X-ray 2.0Å R=1-166.
2VH5 X-ray 2.7Å R=1-166.
2X1V X-ray 1.7Å A=1-166.
3DDC X-ray 1.8Å A=1-166.
3I3S X-ray 1.3Å R=1-166.
3K8Y X-ray 1.3Å A=1-166.
3K9L X-ray 1.8Å A/B/C=1-166.
3K9N X-ray 2.0Å A=1-166.
3KKM X-ray 1.7Å A=1-166.
3KKN X-ray 2.0Å A=1-166.
3KUD X-ray 2.1Å A=1-166.
3L8Y X-ray 2.0Å A=1-166.
3L8Z X-ray 1.4Å A=1-166.
3LBH X-ray 1.8Å A=1-166.
3LBI X-ray 2.0Å A=1-166.
3LBN X-ray 1.8Å A=1-166.
3LO5 X-ray 2.5Å A/C/E=1-166.
3OIU X-ray 1.3Å A=1-166.
3OIV X-ray 1.8Å A=1-166.
3OIW X-ray 1.3Å A=1-166.
3RRY X-ray 1.6Å A=1-166.
3RRZ X-ray 1.6Å A=1-166.
3RS0 X-ray 1.4Å A=1-166.
3RS2 X-ray 1.8Å A=1-166.
3RS3 X-ray 1.5Å A=1-166.
3RS4 X-ray 1.7Å A=1-166.
3RS5 X-ray 1.6Å A=1-166.
3RS7 X-ray 1.7Å A=1-166.
3RSL X-ray 1.7Å A=1-166.
3RSO X-ray 1.6Å A=1-166.
3TGP X-ray 1.3Å A=1-166.
421P X-ray 2.2Å A=1-166.
4DLR X-ray 1.3Å A=1-166.
4DLS X-ray 1.8Å A=1-166.
4DLT X-ray 1.7Å A=1-166.
4DLU X-ray 1.6Å A=1-166.
4DLV X-ray 1.5Å A=1-166.
4DLW X-ray 1.7Å A=1-166.
4DLX X-ray 1.7Å A=1-166.
4DLY X-ray 1.5Å A=1-166.
4DLZ X-ray 1.6Å A=1-166.
4DST X-ray 2.3Å A=2-167.
4DSU X-ray 1.7Å A=2-167.
4EFL X-ray 1.9Å A=1-166.
4EFM X-ray 1.9Å A=1-166.
4EFN X-ray 2.3Å A=1-166.
4G0N X-ray 2.4Å A=1-166.
4G3X X-ray 3.2Å A=1-166.
4K81 X-ray 2.4Å B/D/F/H=1-166.
4L9S X-ray 1.6Å A=1-166.
4L9W X-ray 1.9Å A=1-166.
4NYI X-ray 2.9Å Q/R=1-166.
4NYJ X-ray 2.8Å Q/R=1-166.
4NYM X-ray 3.5Å Q/R=1-166.
4Q21 X-ray 2.0Å A=1-189.
4RSG Neutron 1.9Å A=1-166.
4URU X-ray 2.8Å R=1-166.
4URV X-ray 2.5Å R=1-166.
4URW X-ray 2.7Å R=1-166.
4URX X-ray 2.4Å R=1-166.
4URY X-ray 2.4Å R=1-166.
4URZ X-ray 2.2Å R=1-166.
4US0 X-ray 2.1Å R=1-166.
4US1 X-ray 2.6Å R=1-166.
4US2 X-ray 2.4Å R=1-166.
4XVQ X-ray 1.8Å A=1-166.
4XVR X-ray 2.0Å A=1-166.
521P X-ray 2.6Å A=1-166.
5B2Z X-ray 1.5Å A=1-166.
5B30 X-ray 1.6Å A=1-166.
5P21 X-ray 1.3Å A=1-166.
621P X-ray 2.4Å A=1-166.
6Q21 X-ray 1.9Å A/B/C/D=1-171.
721P X-ray 2.0Å A=1-166.
821P X-ray 1.5Å A=1-166.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef synergizes with 10748182
Envelope surface glycoprotein gp160; precursor inactivates 9344703
Tat activates 16436505
Envelope surface glycoprotein gp160; precursor activates 9269777
Nef activates 11958689

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01521 EGFR tyrosine kinase inhibitor resistance - Homo sapiens (human)
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04012 ErbB signaling pathway - Homo sapiens (human)
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04071 Sphingolipid signaling pathway - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04150 mTOR signaling pathway - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04211 Longevity regulating pathway - Homo sapiens (human)
hsa04213 Longevity regulating pathway - multiple species - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04370 VEGF signaling pathway - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04540 Gap junction - Homo sapiens (human)
hsa04550 Signaling pathways regulating pluripotency of stem cells - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04662 B cell receptor signaling pathway - Homo sapiens (human)
hsa04664 Fc epsilon RI signaling pathway - Homo sapiens (human)
hsa04720 Long-term potentiation - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)
hsa04725 Cholinergic synapse - Homo sapiens (human)
hsa04726 Serotonergic synapse - Homo sapiens (human)
hsa04730 Long-term depression - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa04917 Prolactin signaling pathway - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04933 AGE-RAGE signaling pathway in diabetic complications - Homo sapiens (human)
hsa05034 Alcoholism - Homo sapiens (human)
hsa05160 Hepatitis C - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05211 Renal cell carcinoma - Homo sapiens (human)
hsa05213 Endometrial cancer - Homo sapiens (human)
hsa05214 Glioma - Homo sapiens (human)
hsa05215 Prostate cancer - Homo sapiens (human)
hsa05216 Thyroid cancer - Homo sapiens (human)
hsa05218 Melanoma - Homo sapiens (human)
hsa05219 Bladder cancer - Homo sapiens (human)
hsa05220 Chronic myeloid leukemia - Homo sapiens (human)
hsa05221 Acute myeloid leukemia - Homo sapiens (human)
hsa05223 Non-small cell lung cancer - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)
hsa05230 Central carbon metabolism in cancer - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)