Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008760
UniProt IDP07711
Primary gene name(s)CTSL
Synonym gene name(s)CTSL1
Protein nameCathepsin L1
Protein functionImportant for the overall degradation of proteins in lysosomes.
Subcellular locationLysosome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P07711
Gene Ontology
(Biological Process)
Complete annatation
adaptive immune response [GO:0002250];
antigen processing and presentation [GO:0019882];
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
cellular response to thyroid hormone stimulus [GO:0097067];
collagen catabolic process [GO:0030574];
extracellular matrix disassembly [GO:0022617];
macrophage apoptotic process [GO:0071888];
proteolysis [GO:0006508];
proteolysis involved in cellular protein catabolic process [GO:0051603];
toll-like receptor signaling pathway [GO:0002224]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interaction107894
Phylogenetic treeP07711
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.574379157010810.01647876964863830.0396880818383311
AZA vs. DISU0.05445205945261960.9364735674777870.99506396880143
AZA vs. IL7-0.3811953331593570.6196159260277750.999311006273513
AZA vs. SAHA-0.8705523919687230.2306753646749030.600864082790649
DISU vs. CD31.614085046392340.005879080033341370.0185905710976689
DISU vs. IL7-0.4417232537217780.539155272131850.848177856600662
DISU vs. SAHA-0.9281506028519030.1653744947881550.525183368823063
DMSO vs. AZA0.06299713900131570.9312635978326021
DMSO vs. CD31.606896134436270.0118862523938610.0288930756173348
DMSO vs. DISU0.001117059136819250.9986779523107580.999672981821529
DMSO vs. IL7-0.4341939326080610.5668520574170920.903127598508334
DMSO vs. SAHA-0.9402084706798070.1922218030560430.52778791276357
HIV vs. Mock in Activation-1.253747455653010.05044694088086880.999983755607037
HIV vs. Mock in Latency-0.6975542365573930.1549647942181790.999834320637052
IL7 vs. CD31.209713686844680.08243699464873090.163131524266814
SAHA vs. CD30.6723815605104870.2697159921568650.381797858520003
SAHA vs. IL7-0.4983989334007350.5183338746850570.741376300123593
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 1.218 2.53E-11 5.34E-09
Infected vs. Bystander 1.239 1.79E-11 2.26E-09
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.71997 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03661 Cysteinesulfonic Acid experimental unknown unknown
DB07477 BIPHENYL-4-YL-ACETALDEHYDE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1CJL X-ray 2.2Å A=22-333.
1CS8 X-ray 1.8Å A=19-333.
1ICF X-ray 2.0Å A/C=114-288# B/D=292-333.
1MHW X-ray 1.9Å A/B=114-288# C/D=292-333.
2NQD X-ray 1.7Å B=113-333.
2VHS X-ray 1.5Å A/B/C/D=114-285# A/B/C/D=294-333.
2XU1 X-ray 1.4Å A/B/C/D=114-333.
2XU3 X-ray 0.9Å A=114-333.
2XU4 X-ray 1.1Å A=114-333.
2XU5 X-ray 1.6Å A=114-333.
2YJ2 X-ray 1.1Å A=114-333.
2YJ8 X-ray 1.3Å A=114-333.
2YJ9 X-ray 1.3Å A=114-333.
2YJB X-ray 1.4Å A=114-333.
2YJC X-ray 1.1Å A=114-333.
3BC3 X-ray 2.2Å A/B=114-333.
3H89 X-ray 2.5Å A/B/C/D/E/F=114-333.
3H8B X-ray 1.8Å A/B/C/D/E/F=114-333.
3H8C X-ray 2.5Å A/B=114-333.
3HHA X-ray 1.2Å A/B/C/D=114-333.
3HWN X-ray 2.3Å A/B/C/D=76-333.
3IV2 X-ray 2.2Å A/B=114-333.
3K24 X-ray 2.5Å A/B=114-333.
3KSE X-ray 1.7Å A/B/C=114-333.
3OF8 X-ray 2.2Å A=113-333.
3OF9 X-ray 1.7Å A=113-333.
4AXL X-ray 1.9Å A=114-333.
4AXM X-ray 2.8Å A/B/F/I/L/O=114-333.
5F02 X-ray 1.4Å A=114-333.
5I4H X-ray 1.4Å A=113-218# B=222-333.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 cleaved by 19939935

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05323 Rheumatoid arthritis - Homo sapiens (human)