Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008680
UniProt IDP53677
Primary gene name(s)AP3M2
Synonym gene name(s)unknown
Protein nameAP-3 complex subunit mu-2
Protein functionPart of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Subcellular locationGolgi apparatus. Cytoplasmic vesicle membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P53677
Gene Ontology
(Biological Process)
Complete annatation
anterograde axonal transport [GO:0008089];
anterograde synaptic vesicle transport [GO:0048490];
intracellular protein transport [GO:0006886]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
AP-type membrane coat adaptor complex [GO:0030119];
axon cytoplasm [GO:1904115];
clathrin adaptor complex [GO:0030131];
cytoplasmic vesicle membrane [GO:0030659];
Golgi apparatus [GO:0005794]
Protein-protein interaction116147
Phylogenetic treeP53677
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4725764522770870.1611242521992960.257498648204097
AZA vs. DISU-0.1111943142475810.662189350747120.961528862271377
AZA vs. IL70.3909892128718680.04301630695904960.626458738931163
AZA vs. SAHA0.5269909904800340.03206290498170250.206987678669886
DISU vs. CD30.3486962232232350.3883268379996440.522833043587587
DISU vs. IL70.4931171051997850.05234616239312910.302112709830176
DISU vs. SAHA0.639719488909730.03450298381606490.22510583077572
DMSO vs. AZA0.09208071741720780.5849566797526931
DMSO vs. CD30.5516842501660030.1073872405673790.181175093347224
DMSO vs. DISU0.2010267551413870.412338235086180.871981100176954
DMSO vs. IL70.3062373597888110.08999769991696760.55671535750853
DMSO vs. SAHA0.4287450996335280.07050492515157770.30942915753041
HIV vs. Mock in Activation-0.09384383626965080.9029569968219040.999983755607037
HIV vs. Mock in Latency0.06006780633175130.717572319512060.999834320637052
IL7 vs. CD30.8716385122819020.009118736541640190.0275921289888934
SAHA vs. CD30.9751006552012780.01630256043563540.0387813079344949
SAHA vs. IL70.132531827610550.5885547821409940.790256551457016
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.3368 0.0337

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.179536 0.25276
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor interacts with 9400603
Envelope surface glycoprotein gp160; precursor co-localizes with 18076669
matrix co-localizes with 18076669
Pr55(Gag) co-localizes with 18076669
Envelope transmembrane glycoprotein gp41 interacts with 9400603

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)