Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008499
UniProt IDQ9UK23
Primary gene name(s)NAGPA
Synonym gene name(s)unknown
Protein nameN-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Protein functionCatalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases. {ECO:0000269|PubMed:23572527}.
Subcellular locationGolgi apparatus, Golgi stack membrane {ECO:0000269|PubMed:12058031};
Single-pass type I membrane protein {ECO:0000269|PubMed:12058031}. Golgi apparatus, trans-Golgi network {ECO:0000269|PubMed:12058031}. Note=Cis/medial Golgi.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UK23
Gene Ontology
(Biological Process)
Complete annatation
carbohydrate metabolic process [GO:0005975];
cellular protein modification process [GO:0006464];
lysosome organization [GO:0007040];
protein glycosylation [GO:0006486];
protein targeting to lysosome [GO:0006622];
secretion of lysosomal enzymes [GO:0033299]
Gene Ontology
(Molecular Function)
Complete annatation
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity [GO:0003944]
Gene Ontology
(Cellular Component)
Complete annatation
Golgi cisterna membrane [GO:0032580];
integral component of membrane [GO:0016021]
Protein-protein interaction119351
Phylogenetic treeQ9UK23
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4277453010701450.1958543562663190.300327282291785
AZA vs. DISU0.1228468870037960.6307775424806870.960057478314726
AZA vs. IL7-0.1527609232490320.4759593279629990.999311006273513
AZA vs. SAHA-0.820431648582190.06502681170310040.313002308824397
DISU vs. CD30.5373844659427710.1414774621730490.244736202065245
DISU vs. IL7-0.2855190540005430.2625941605442970.648476910709538
DISU vs. SAHA-0.9396535075288920.03117183376412280.211993982038213
DMSO vs. AZA0.05795624643214570.7367313877838061
DMSO vs. CD30.4727536284364090.1437661430944510.229431879842999
DMSO vs. DISU-0.06702355493988870.7856595690572180.973114958962133
DMSO vs. IL7-0.2027623422239840.2704865496054960.768786747755014
DMSO vs. SAHA-0.8821921414377820.03447210241902930.201994189563449
HIV vs. Mock in Activation0.1206212611013660.8482620632191210.999983755607037
HIV vs. Mock in Latency0.0270719031408760.8734475841375680.999834320637052
IL7 vs. CD30.2791530968821160.390728577655290.527586836065411
SAHA vs. CD3-0.4148732001231460.3674893304496790.484169775820571
SAHA vs. IL7-0.669054025542630.1279822070650690.332850895324164
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.5691 0.01301

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.453169 0.084043
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00141 N-Acetyl-D-glucosamine approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)