Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008306
UniProt IDQ16236
Primary gene name(s)NFE2L2
Synonym gene name(s)NRF2
Protein nameNuclear factor erythroid 2-related factor 2
Protein functionTranscription activator that binds to antioxidant response, ARE elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. {ECO:0000269|PubMed:11035812}.
Subcellular locationCytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q16236
Gene Ontology
(Biological Process)
Complete annatation
aging [GO:0007568];
cell redox homeostasis [GO:0045454];
cellular response to drug [GO:0035690];
cellular response to fluid shear stress [GO:0071498];
cellular response to glucose starvation [GO:0042149];
cellular response to hydrogen peroxide [GO:0070301];
cellular response to hypoxia [GO:0071456];
cellular response to laminar fluid shear stress [GO:0071499];
cellular response to oxidative stress [GO:0034599];
cellular response to tumor necrosis factor [GO:0071356];
endoplasmic reticulum unfolded protein response [GO:0030968];
inflammatory response [GO:0006954];
negative regulation of cardiac muscle cell apoptotic process [GO:0010667];
negative regulation of endothelial cell apoptotic process [GO:2000352];
negative regulation of hematopoietic stem cell differentiation [GO:1902037];
negative regulation of hydrogen peroxide-induced cell death [GO:1903206];
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [GO:1902176];
negative regulation of vascular associated smooth muscle cell migration [GO:1904753];
PERK-mediated unfolded protein response [GO:0036499];
positive regulation of angiogenesis [GO:0045766];
positive regulation of blood coagulation [GO:0030194];
positive regulation of blood vessel endothelial cell migration [GO:0043536];
positive regulation of ER-associated ubiquitin-dependent protein catabolic process [GO:1903071];
positive regulation of gene expression [GO:0010628];
positive regulation of glucose import [GO:0046326];
positive regulation of glutathione biosynthetic process [GO:1903788];
positive regulation of neuron projection development [GO:0010976];
positive regulation of reactive oxygen species metabolic process [GO:2000379];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress [GO:0036091];
positive regulation of transcription from RNA polymerase II promoter in response to stress [GO:0036003];
proteasomal ubiquitin-independent protein catabolic process [GO:0010499];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein ubiquitination [GO:0016567];
regulation of embryonic development [GO:0045995];
regulation of removal of superoxide radicals [GO:2000121];
transcription from RNA polymerase II promoter [GO:0006366]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
protein domain specific binding [GO:0019904];
RNA polymerase II activating transcription factor binding [GO:0001102];
RNA polymerase II distal enhancer sequence-specific DNA binding [GO:0000980];
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding [GO:0001205];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
transcription regulatory region DNA binding [GO:0044212];
transcription regulatory region sequence-specific DNA binding [GO:0000976]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
chromatin [GO:0000785];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
protein-DNA complex [GO:0032993]
Protein-protein interaction110852
Phylogenetic treeQ16236
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6697778328275250.04155888662083570.085442032348825
AZA vs. DISU0.232732065038350.3574887016728270.892482504096917
AZA vs. IL70.03032439937041820.874557618033980.999311006273513
AZA vs. SAHA-0.1641124915438140.5008783247395280.821311269581892
DISU vs. CD3-0.4499250048209120.2161350588918070.338214458396667
DISU vs. IL7-0.2114780136315520.4009830946662850.764199766383597
DISU vs. SAHA-0.3956375275763670.1751673455089910.539094764992036
DMSO vs. AZA-0.06788771497442490.6851144887422771
DMSO vs. CD3-0.748756424477340.01974573588629150.044352096485311
DMSO vs. DISU-0.302436652219010.2154763864177550.727262966028124
DMSO vs. IL70.1054284443640840.5574090100602630.900949551185115
DMSO vs. SAHA-0.1030893525693780.6616571532208660.893649775454289
HIV vs. Mock in Activation-0.1393462012740910.8226206913894620.999983755607037
HIV vs. Mock in Latency0.03411365910360680.8362925168590270.999834320637052
IL7 vs. CD3-0.6315843967649050.05014460989146730.110002356394579
SAHA vs. CD3-0.8588647901236170.01567083833728780.0374634120946334
SAHA vs. IL7-0.1982119695567150.4155136882958620.660214328804873
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0489741 0.790738
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201146_at 1.86 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2FLU X-ray 1.5Å P=69-84.
2LZ1 NMR - A=445-523.
3ZGC X-ray 2.2Å C=76-82.
4IFL X-ray 1.8Å P=69-84.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 upregulates 21756955
Envelope surface glycoprotein gp120 regulated by 22528837
Tat interacts with 24414799

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)