Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008271
UniProt IDQ5SW96
Primary gene name(s)LDLRAP1
Synonym gene name(s)ARH
Protein nameLow density lipoprotein receptor adapter protein 1
Protein functionAdapter protein, clathrin-associated sorting protein, CLASP required for efficient endocytosis of the LDL receptor, LDLR in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells, fibroblasts. May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. {ECO:0000269|PubMed:15728179}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:12451172}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q5SW96
Gene Ontology
(Biological Process)
Complete annatation
amyloid precursor protein metabolic process [GO:0042982];
cholesterol homeostasis [GO:0042632];
cholesterol metabolic process [GO:0008203];
low-density lipoprotein particle clearance [GO:0034383];
positive regulation of cholesterol metabolic process [GO:0090205];
positive regulation of receptor-mediated endocytosis [GO:0048260];
positive regulation of receptor-mediated endocytosis involved in cholesterol transport [GO:1905602];
receptor internalization [GO:0031623];
receptor-mediated endocytosis [GO:0006898];
receptor-mediated endocytosis involved in cholesterol transport [GO:0090118];
regulation of establishment of protein localization to plasma membrane [GO:0090003];
regulation of protein binding [GO:0043393];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
AP-2 adaptor complex binding [GO:0035612];
beta-amyloid binding [GO:0001540];
clathrin adaptor activity [GO:0035615];
clathrin binding [GO:0030276];
low-density lipoprotein particle receptor binding [GO:0050750];
phosphatidylinositol-4,5-bisphosphate binding [GO:0005546];
phosphotyrosine binding [GO:0001784];
receptor signaling complex scaffold activity [GO:0030159];
signaling adaptor activity [GO:0035591]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
basal plasma membrane [GO:0009925];
cytoplasmic side of plasma membrane [GO:0009898];
cytosol [GO:0005829];
early endosome [GO:0005769];
neurofilament [GO:0005883];
recycling endosome [GO:0055037]
Protein-protein interaction117561
Phylogenetic treeQ5SW96
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.730757198977451.54389324324988e-071.4188755464794e-06
AZA vs. DISU-0.1469867707622510.5613685697043120.950719067949265
AZA vs. IL7-0.4018848483928440.03741901469068910.593077611390911
AZA vs. SAHA-0.4893360610319250.04591012673738260.256839199796044
DISU vs. CD32.571346180482728.40261946244425e-078.30564496897604e-06
DISU vs. IL7-0.263962251097270.2958366599587660.680028801091181
DISU vs. SAHA-0.3410859014614870.2434156886299220.622034267636486
DMSO vs. AZA0.07432965303669410.6580139901133151
DMSO vs. CD32.788208838807727.73545908439033e-086.93658875632978e-07
DMSO vs. DISU0.2178666910656490.3723945048386820.852590870876848
DMSO vs. IL7-0.4681579705706390.009538468955776480.203671982306417
DMSO vs. SAHA-0.568736012782930.01632462489412890.123819034614704
HIV vs. Mock in Activation0.05698036979335260.95776124644280.999983755607037
HIV vs. Mock in Latency-0.1801457869903010.2781934891144970.999834320637052
IL7 vs. CD32.336944842635593.28529029614e-062.84592992147806e-05
SAHA vs. CD32.216877100887649.58165001496702e-066.05685678378998e-05
SAHA vs. IL7-0.09175505632824570.7068224889436130.865386793237168
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock -1.193 1.12E-13 1.30E-10
Infected vs. Bystander -1.298 1.79E-14 1.92E-11
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1 0.529303358 -1.4 0.011639275 -1.8 0.003751611
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.942544 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
221790_s_at 2.06 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2G30 X-ray 1.6Å P=252-267.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)