Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008225
UniProt IDQ9NZN3
Primary gene name(s)EHD3
Synonym gene name(s)EHD2, PAST3
Protein nameEH domain-containing protein 3
Protein functionATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis, PubMed:25686250. In vitro causes tubulation of endocytic membranes, PubMed:24019528. Binding to phosphatidic acid induces its membrane tubulation activity, By similarity. Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment, ERC, retrograde early endosome to Golgi, and endosome to plasma membrane, rapid recycling protein transport. Involved in the regulation of Golgi maintenance and morphology, PubMed:16251358, PubMed:17233914, PubMed:19139087, PubMed:23781025. Involved in the recycling of internalized D1 dopamine receptor, PubMed:21791287. Plays a role in cardiac protein trafficking probably implicating ANK2, PubMed:20489164. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction, By similarity. In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function, By similarity. Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance, PubMed:23781025. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing, By similarity. Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with EHD1, PubMed:25686250. {ECO:0000250|UniProtKB:Q9QXY6, ECO:0000269|PubMed:16251358, ECO:0000269|PubMed:17233914, ECO:0000269|PubMed:19139087, ECO:0000269|PubMed:21791287, ECO:0000269|PubMed:23781025, ECO:0000269|PubMed:24019528, ECO:0000269|PubMed:25686250, ECO:0000305|PubMed:20489164}.
Subcellular locationRecycling endosome membrane {ECO:0000269|PubMed:17233914};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Cell membrane {ECO:0000269|PubMed:17233914};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Cell projection, cilium membrane {ECO:0000269|PubMed:25686250};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Note=Localizes to the ciliary pocket from where the cilium protrudes, PubMed:25686250. Colocalizes with RAB8A and MYO5B to a cytoplasmic tubular network devoid of RAB11A, By similarity. Colocalizes with ANK2 in myocyte perinuclear region, PubMed:20489164. Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals, By similarity. {ECO:0000250|UniProtKB:Q9QXY6, ECO:0000269|PubMed:20489164, ECO:0000269|PubMed:25686250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NZN3
Gene Ontology
(Biological Process)
Complete annatation
blood coagulation [GO:0007596];
cilium assembly [GO:0060271];
early endosome to Golgi transport [GO:0034498];
endocytic recycling [GO:0032456];
Golgi to lysosome transport [GO:0090160];
positive regulation of voltage-gated calcium channel activity [GO:1901387];
protein homooligomerization [GO:0051260];
protein targeting to plasma membrane [GO:0072661];
receptor recycling [GO:0001881];
regulation of cardiac conduction [GO:1903779];
regulation of cardiac muscle cell membrane potential [GO:0086036];
regulation of cardiac muscle contraction [GO:0055117];
regulation of Golgi organization [GO:1903358]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium ion binding [GO:0005509];
GTP binding [GO:0005525];
nucleic acid binding [GO:0003676]
Gene Ontology
(Cellular Component)
Complete annatation
ciliary pocket membrane [GO:0020018];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endocytic vesicle [GO:0030139];
endosome membrane [GO:0010008];
focal adhesion [GO:0005925];
myelin sheath [GO:0043209];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
recycling endosome membrane [GO:0055038]
Protein-protein interaction119055
Phylogenetic treeQ9NZN3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1249822937266960.7145654593646280.797641403455098
AZA vs. DISU-0.09216801844571240.7383347013037680.974746653306445
AZA vs. IL70.02834187410888320.8999167290898730.999311006273513
AZA vs. SAHA-0.4195125049652330.5617417182085310.851690713484642
DISU vs. CD30.02172179055872670.9538141736251570.970096137455342
DISU vs. IL70.1087657884932980.7040631482204850.923899021537588
DISU vs. SAHA-0.3235023035587840.665171777135840.898199431342554
DMSO vs. AZA-0.009007366299352450.9706962405272571
DMSO vs. CD30.1109887549269480.7393256557623830.811317015662051
DMSO vs. DISU0.08330556861442850.7854577522788150.973114958962133
DMSO vs. IL70.04246031426638240.8703658918781470.971598458676035
DMSO vs. SAHA-0.4170532151747830.5724176137428510.850947510033391
HIV vs. Mock in Activation0.5438691690391830.3956252561665180.999983755607037
HIV vs. Mock in Latency0.1319527909428840.5098059379263810.999834320637052
IL7 vs. CD30.1566147712902410.6389183071567790.748516683023017
SAHA vs. CD3-0.3151040320482990.6569798433647330.744412499211017
SAHA vs. IL7-0.4470753505026110.5330742614385780.752604796939695
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.558693 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
218935_at 2.36 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)