Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008198
UniProt IDO60729
Primary gene name(s)CDC14B
Synonym gene name(s)unknown
Protein nameDual specificity protein phosphatase CDC14B
Protein functionDual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome, APC/C. Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases. {ECO:0000269|PubMed:17488717, ECO:0000269|PubMed:18662541, ECO:0000269|PubMed:9367992}.
Subcellular locationNucleus, nucleolus. Nucleus, nucleoplasm. Note=Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60729
Gene Ontology
(Biological Process)
Complete annatation
DNA repair [GO:0006281];
G2 DNA damage checkpoint [GO:0031572];
positive regulation of ubiquitin protein ligase activity [GO:1904668];
protein dephosphorylation [GO:0006470]
Gene Ontology
(Molecular Function)
Complete annatation
protein serine/threonine phosphatase activity [GO:0004722];
protein tyrosine/serine/threonine phosphatase activity [GO:0008138];
protein tyrosine phosphatase activity [GO:0004725]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear membrane [GO:0031965];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction114125
Phylogenetic treeO60729
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1915626572220340.5605987807207630.67031692368293
AZA vs. DISU-0.02512518268568890.9216685666549350.994889252656529
AZA vs. IL7-0.712797880911570.001043032702179430.0814592327458574
AZA vs. SAHA0.5768663750695580.01954457296785230.152020068584832
DISU vs. CD3-0.2285642850072160.5295385992439210.651068550136329
DISU vs. IL7-0.6980481299223880.006504181284734620.088333813394031
DISU vs. SAHA0.6036901525264060.03989602770642010.245060054655281
DMSO vs. AZA0.01789452012087850.9170181695694241
DMSO vs. CD3-0.1835815464318350.5678598729746670.669076254212683
DMSO vs. DISU0.04172624945187640.8655541650885720.98490208197302
DMSO vs. IL7-0.7238215864574670.0009808520096072910.0560088789576891
DMSO vs. SAHA0.5528343995486050.02050426036421390.144082886297535
HIV vs. Mock in Activation0.772226567464820.2183814037280650.999983755607037
HIV vs. Mock in Latency0.5889325412159230.002960694912970130.14396617780036
IL7 vs. CD3-0.8999910685433220.00577765618299630.0189359886162869
SAHA vs. CD30.3612575485480740.3081954324211030.421959222911422
SAHA vs. IL71.286147809847852.77881069377095e-071.52875154736873e-05
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.887737 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1OHC X-ray 2.5Å A=39-386.
1OHD X-ray 2.6Å A=39-386.
1OHE X-ray 2.2Å A=39-386.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)