Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008175
UniProt IDP10415
Primary gene name(s)BCL2
Synonym gene name(s)unknown
Protein nameApoptosis regulator Bcl-2
Protein functionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor, APAF-1. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release, PubMed:17418785. {ECO:0000269|PubMed:17418785, ECO:0000269|PubMed:18570871}.
Subcellular locationMitochondrion outer membrane {ECO:0000269|PubMed:2250705};
Single-pass membrane protein {ECO:0000269|PubMed:2250705}. Nucleus membrane {ECO:0000269|PubMed:2250705};
Single-pass membrane protein {ECO:0000269|PubMed:2250705}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:2250705};
Single-pass membrane protein {ECO:0000269|PubMed:2250705}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P10415
Gene Ontology
(Biological Process)
Complete annatation
actin filament organization [GO:0007015];
apoptotic process [GO:0006915];
axonogenesis [GO:0007409];
axon regeneration [GO:0031103];
B cell homeostasis [GO:0001782];
B cell lineage commitment [GO:0002326];
B cell proliferation [GO:0042100];
B cell receptor signaling pathway [GO:0050853];
behavioral fear response [GO:0001662];
branching involved in ureteric bud morphogenesis [GO:0001658];
CD8-positive, alpha-beta T cell lineage commitment [GO:0043375];
cell aging [GO:0007569];
cell growth [GO:0016049];
cellular response to DNA damage stimulus [GO:0006974];
cellular response to glucose starvation [GO:0042149];
cellular response to hypoxia [GO:0071456];
cellular response to organic substance [GO:0071310];
cochlear nucleus development [GO:0021747];
defense response to virus [GO:0051607];
digestive tract morphogenesis [GO:0048546];
ear development [GO:0043583];
endoplasmic reticulum calcium ion homeostasis [GO:0032469];
extrinsic apoptotic signaling pathway in absence of ligand [GO:0097192];
extrinsic apoptotic signaling pathway via death domain receptors [GO:0008625];
female pregnancy [GO:0007565];
focal adhesion assembly [GO:0048041];
gland morphogenesis [GO:0022612];
glomerulus development [GO:0032835];
hair follicle morphogenesis [GO:0031069];
homeostasis of number of cells within a tissue [GO:0048873];
humoral immune response [GO:0006959];
intrinsic apoptotic signaling pathway in response to DNA damage [GO:0008630];
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [GO:0070059];
intrinsic apoptotic signaling pathway in response to oxidative stress [GO:0008631];
lymphoid progenitor cell differentiation [GO:0002320];
male gonad development [GO:0008584];
melanin metabolic process [GO:0006582];
melanocyte differentiation [GO:0030318];
mesenchymal cell development [GO:0014031];
metanephros development [GO:0001656];
negative regulation of anoikis [GO:2000811];
negative regulation of apoptotic process [GO:0043066];
negative regulation of apoptotic signaling pathway [GO:2001234];
negative regulation of autophagy [GO:0010507];
negative regulation of calcium ion transport into cytosol [GO:0010523];
negative regulation of cell growth [GO:0030308];
negative regulation of cell migration [GO:0030336];
negative regulation of cellular pH reduction [GO:0032848];
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [GO:2001240];
negative regulation of G1/S transition of mitotic cell cycle [GO:2000134];
negative regulation of intrinsic apoptotic signaling pathway [GO:2001243];
negative regulation of mitochondrial depolarization [GO:0051902];
negative regulation of myeloid cell apoptotic process [GO:0033033];
negative regulation of neuron apoptotic process [GO:0043524];
negative regulation of ossification [GO:0030279];
negative regulation of osteoblast proliferation [GO:0033689];
negative regulation of reactive oxygen species metabolic process [GO:2000378];
negative regulation of retinal cell programmed cell death [GO:0046671];
neuron apoptotic process [GO:0051402];
oocyte development [GO:0048599];
organ growth [GO:0035265];
ossification [GO:0001503];
ovarian follicle development [GO:0001541];
peptidyl-serine phosphorylation [GO:0018105];
peptidyl-threonine phosphorylation [GO:0018107];
pigment granule organization [GO:0048753];
positive regulation of B cell proliferation [GO:0030890];
positive regulation of catalytic activity [GO:0043085];
positive regulation of cell growth [GO:0030307];
positive regulation of intrinsic apoptotic signaling pathway [GO:2001244];
positive regulation of melanocyte differentiation [GO:0045636];
positive regulation of multicellular organism growth [GO:0040018];
positive regulation of neuron maturation [GO:0014042];
positive regulation of peptidyl-serine phosphorylation [GO:0033138];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
positive regulation of skeletal muscle fiber development [GO:0048743];
positive regulation of smooth muscle cell migration [GO:0014911];
post-embryonic development [GO:0009791];
protein dephosphorylation [GO:0006470];
protein polyubiquitination [GO:0000209];
reactive oxygen species metabolic process [GO:0072593];
regulation of calcium ion transport [GO:0051924];
regulation of cell-matrix adhesion [GO:0001952];
regulation of gene expression [GO:0010468];
regulation of glycoprotein biosynthetic process [GO:0010559];
regulation of mitochondrial membrane permeability [GO:0046902];
regulation of mitochondrial membrane potential [GO:0051881];
regulation of nitrogen utilization [GO:0006808];
regulation of protein heterodimerization activity [GO:0043497];
regulation of protein homodimerization activity [GO:0043496];
regulation of protein stability [GO:0031647];
regulation of transmembrane transporter activity [GO:0022898];
regulation of viral genome replication [GO:0045069];
release of cytochrome c from mitochondria [GO:0001836];
renal system process [GO:0003014];
response to cytokine [GO:0034097];
response to drug [GO:0042493];
response to gamma radiation [GO:0010332];
response to glucocorticoid [GO:0051384];
response to hydrogen peroxide [GO:0042542];
response to iron ion [GO:0010039];
response to ischemia [GO:0002931];
response to nicotine [GO:0035094];
response to radiation [GO:0009314];
response to toxic substance [GO:0009636];
response to UV-B [GO:0010224];
spleen development [GO:0048536];
T cell differentiation in thymus [GO:0033077];
T cell homeostasis [GO:0043029];
thymus development [GO:0048538]
Gene Ontology
(Molecular Function)
Complete annatation
BH3 domain binding [GO:0051434];
channel activity [GO:0015267];
channel inhibitor activity [GO:0016248];
identical protein binding [GO:0042802];
protease binding [GO:0002020];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803];
sequence-specific DNA binding [GO:0043565];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
membrane [GO:0016020];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739];
myelin sheath [GO:0043209];
nuclear membrane [GO:0031965];
nucleus [GO:0005634];
pore complex [GO:0046930]
Protein-protein interaction107068
Phylogenetic treeP10415
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.743312331729282.34943580479374e-072.08420814890237e-06
AZA vs. DISU-0.3021751261702590.2316253009310520.818071474852885
AZA vs. IL70.4547052289475770.01783871147987640.436597117879048
AZA vs. SAHA0.04133920496477840.8650893224006580.968340596907961
DISU vs. CD31.430447953092540.0001078099257908470.000605712568531953
DISU vs. IL70.7474531590674840.003090962254647780.0561977966986692
DISU vs. SAHA0.3451431364993620.2362186053639360.614331423889539
DMSO vs. AZA-0.01509738595724430.927868397752771
DMSO vs. CD31.720001348962631.68701014713335e-071.41823704612522e-06
DMSO vs. DISU0.2861621113132030.2400704336325620.757085339999873
DMSO vs. IL70.4765397601284880.00793471929216960.185638917408722
DMSO vs. SAHA0.04952596069841360.8332480197709970.954874068570649
HIV vs. Mock in Activation-0.05467975940566760.9303323474527630.999983755607037
HIV vs. Mock in Latency-0.2813740078706980.6352642084722810.999834320637052
IL7 vs. CD32.205227937267534.91012785985845e-111.09807651679001e-09
SAHA vs. CD31.761189897812531.37150913459561e-061.07537162634525e-05
SAHA vs. IL7-0.415591302193770.08784635992190020.265582837838492
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.9 0.000548014 1.4 0.021596602 1.7 0.119287129
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.424977 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category