Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0008125
UniProt IDQ01094
Primary gene name(s)E2F1
Synonym gene name(s)RBBP3
Protein nameTranscription factor E2F1
Protein functionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters, PubMed:20176812. {ECO:0000250|UniProtKB:Q61501, ECO:0000269|PubMed:10675335, ECO:0000269|PubMed:12717439, ECO:0000269|PubMed:17704056, ECO:0000269|PubMed:20176812, ECO:0000269|PubMed:8170954}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q01094
Gene Ontology
(Biological Process)
Complete annatation
anoikis [GO:0043276];
cellular response to fatty acid [GO:0071398];
cellular response to hypoxia [GO:0071456];
cellular response to nerve growth factor stimulus [GO:1990090];
cellular response to xenobiotic stimulus [GO:0071466];
DNA damage checkpoint [GO:0000077];
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
forebrain development [GO:0030900];
intrinsic apoptotic signaling pathway by p53 class mediator [GO:0072332];
intrinsic apoptotic signaling pathway in response to DNA damage [GO:0008630];
lens fiber cell apoptotic process [GO:1990086];
mRNA stabilization [GO:0048255];
negative regulation of DNA binding [GO:0043392];
negative regulation of fat cell differentiation [GO:0045599];
negative regulation of fat cell proliferation [GO:0070345];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
negative regulation of transcription involved in G1/S transition of mitotic cell cycle [GO:0071930];
positive regulation of fibroblast proliferation [GO:0048146];
positive regulation of gene expression [GO:0010628];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of G1/S transition of mitotic cell cycle [GO:2000045];
regulation of transcription, DNA-templated [GO:0006355];
spermatogenesis [GO:0007283];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
core promoter binding [GO:0001047];
DNA binding [GO:0003677];
sequence-specific DNA binding [GO:0043565];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrion [GO:0005739];
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
Rb-E2F complex [GO:0035189]
Protein-protein interaction108201
Phylogenetic treeQ01094
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      Yes - >3 <4 SD (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.969962999919120.02666482823849450.0589976939534041
AZA vs. DISU0.3546759390968350.1714718138703050.754896647862435
AZA vs. IL70.9743382404518580.007348747811419920.273634381687483
AZA vs. SAHA-0.5811262768455480.02169135043161630.16246510262244
DISU vs. CD3-1.630135832355540.05653320589109770.119169563082423
DISU vs. IL70.6117883000576180.1007412760695450.420485068104261
DISU vs. SAHA-0.9340842982841120.001896329453780380.0325137442524733
DMSO vs. AZA0.04823605119765270.8482933826802821
DMSO vs. CD3-1.942082199681810.03068567486453810.0639922054034145
DMSO vs. DISU-0.3107611850132340.2572850064709530.77530194911363
DMSO vs. IL70.9343248681012010.01853604565697940.282187236229219
DMSO vs. SAHA-0.6342271046531010.02122039553082360.147175601033153
HIV vs. Mock in Activation0.05473671458798590.9735041665166470.999983755607037
HIV vs. Mock in Latency-0.5435753662596030.009834563788511330.327254336750596
IL7 vs. CD3-0.9866330313940950.2472827232298230.37821334218601
SAHA vs. CD3-2.576668107478020.004971639587894590.0141952649847616
SAHA vs. IL7-1.560504554484727.18548821827536e-050.00148783034893246
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.517293 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
2028_s_at 1.53 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1H24 X-ray 2.5Å E=87-95.
1O9K X-ray 2.6Å P/Q/R/S=409-426.
2AZE X-ray 2.5Å B=200-301.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat inhibited by 7474112
Envelope surface glycoprotein gp120 activates 17011204
HIV-1 virus replication inhibited by expression of human gene 22082156
Envelope surface glycoprotein gp120 requires 17011204

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04110 Cell cycle - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05212 Pancreatic cancer - Homo sapiens (human)
hsa05214 Glioma - Homo sapiens (human)
hsa05215 Prostate cancer - Homo sapiens (human)
hsa05218 Melanoma - Homo sapiens (human)
hsa05219 Bladder cancer - Homo sapiens (human)
hsa05220 Chronic myeloid leukemia - Homo sapiens (human)
hsa05222 Small cell lung cancer - Homo sapiens (human)
hsa05223 Non-small cell lung cancer - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)