Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007926
UniProt IDQ04917
Primary gene name(s)YWHAH
Synonym gene name(s)YWHA1
Protein name14-3-3 protein eta
Protein functionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. {ECO:0000269|PubMed:12177059}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q04917
Gene Ontology
(Biological Process)
Complete annatation
glucocorticoid catabolic process [GO:0006713];
glucocorticoid receptor signaling pathway [GO:0042921];
intracellular protein transport [GO:0006886];
membrane depolarization during action potential [GO:0086010];
membrane organization [GO:0061024];
negative regulation of dendrite morphogenesis [GO:0050774];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
positive regulation of transcription, DNA-templated [GO:0045893];
regulation of neuron differentiation [GO:0045664];
regulation of sodium ion transmembrane transporter activity [GO:2000649];
regulation of sodium ion transport [GO:0002028];
regulation of synaptic plasticity [GO:0048167];
substantia nigra development [GO:0021762]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
glucocorticoid receptor binding [GO:0035259];
insulin-like growth factor receptor binding [GO:0005159];
ion channel binding [GO:0044325];
protein domain specific binding [GO:0019904];
protein heterodimerization activity [GO:0046982];
sodium channel regulator activity [GO:0017080]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
intercalated disc [GO:0014704];
mitochondrion [GO:0005739];
plasma membrane [GO:0005886]
Protein-protein interaction113365
Phylogenetic treeQ04917
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7122212945880680.03399766036960440.0723121826136535
AZA vs. DISU-0.02753087170039370.9135985362431550.994271142470137
AZA vs. IL70.2649211785597610.169865938634440.941150532286992
AZA vs. SAHA-0.08899564221904050.7158696561448360.92034803561728
DISU vs. CD3-0.7513253713049670.04114451127421170.092279774281724
DISU vs. IL70.2826915574257590.2627000418204890.648476910709538
DISU vs. SAHA-0.05935989592917280.8387736584973270.958116288785572
DMSO vs. AZA-0.01245561257251960.9410383160973671
DMSO vs. CD3-0.7329739485821670.03010273979686210.0630257791356022
DMSO vs. DISU0.01403603572353680.9542494196072120.993828264951078
DMSO vs. IL70.2842712743227990.1153160392937040.603561174685793
DMSO vs. SAHA-0.08286285270597680.7257465544787980.919291168477893
HIV vs. Mock in Activation0.1612480943879960.8230205872202990.999983755607037
HIV vs. Mock in Latency0.2090255900646040.2083296133880970.999834320637052
IL7 vs. CD3-0.4413306151829680.1764010182027610.293314125468309
SAHA vs. CD3-0.8248774597906220.02243570059406390.0507103297512299
SAHA vs. IL7-0.3558367574704890.1449387384779140.359165961501902
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.948698097
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.105116 0.509299
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.01 0.925 0.94 0.885 0.872
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2C63 X-ray 2.1Å A/B/C/D=2-246.
2C74 X-ray 2.7Å A/B=2-246.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr enhanced by 11531413
Vpr complexes with 15142377
Vpr binds 15708996
Vpr inhibits 15265780

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)