Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007902
UniProt IDO14980
Primary gene name(s)XPO1
Synonym gene name(s)CRM1
Protein nameExportin-1
Protein functionMediates the nuclear export of cellular proteins, cargos bearing a leucine-rich nuclear export signal, NES and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form, Ran-GTP. Docking of this complex to the nuclear pore complex, NPC is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP, induced by RANBP1 and RANGAP1, respectively cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. {ECO:0000269|PubMed:14612415, ECO:0000269|PubMed:15574332, ECO:0000269|PubMed:20921223, ECO:0000269|PubMed:9311922, ECO:0000269|PubMed:9323133, ECO:0000269|PubMed:9837918}.
Subcellular locationCytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Note=Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14980
Gene Ontology
(Biological Process)
Complete annatation
intracellular transport of virus [GO:0075733];
mRNA transport [GO:0051028];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
protein export from nucleus [GO:0006611];
protein localization to nucleus [GO:0034504];
regulation of centrosome duplication [GO:0010824];
regulation of mRNA stability [GO:0043488];
regulation of protein catabolic process [GO:0042176];
regulation of protein export from nucleus [GO:0046825];
response to drug [GO:0042493];
ribosomal large subunit export from nucleus [GO:0000055];
ribosomal small subunit export from nucleus [GO:0000056];
ribosomal subunit export from nucleus [GO:0000054];
sister chromatid cohesion [GO:0007062];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
nucleocytoplasmic transporter activity [GO:0005487];
protein transporter activity [GO:0008565];
RNA binding [GO:0003723];
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
annulate lamellae [GO:0005642];
Cajal body [GO:0015030];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
intracellular membrane-bounded organelle [GO:0043231];
intracellular ribonucleoprotein complex [GO:0030529];
kinetochore [GO:0000776];
membrane [GO:0016020];
nuclear envelope [GO:0005635];
nuclear membrane [GO:0031965];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113348
Phylogenetic treeO14980
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.143767545730350.0005476680401736990.00215314101276265
AZA vs. DISU0.3673912981589650.1462329038970560.719729072365598
AZA vs. IL70.2936617371520290.1831957597493110.950055281862931
AZA vs. SAHA-0.0736889317153620.7620483263596440.936500609126469
DISU vs. CD3-0.7901633055098020.0299496198452510.0714449390009992
DISU vs. IL7-0.081977946878130.7546982982796680.941323479325969
DISU vs. SAHA-0.4405965757031930.130800808229860.465477917551882
DMSO vs. AZA-0.04013762327285460.8099278815134741
DMSO vs. CD3-1.196932781947130.000216486635902680.000891572217024021
DMSO vs. DISU-0.4098270822859090.09310356896350690.558542532800995
DMSO vs. IL70.3412741126124030.07938194365195750.529175075205031
DMSO vs. SAHA-0.04108363184679790.8613214344803160.964100255236753
HIV vs. Mock in Activation-0.3185884668693290.6109521892900220.999983755607037
HIV vs. Mock in Latency-0.1814948080112790.2698208530990330.999834320637052
IL7 vs. CD3-0.8404051832909390.009217379231690080.0278535599757948
SAHA vs. CD3-1.244181438839240.0005085627538395920.00198550277211468
SAHA vs. IL7-0.3719714687269950.1263513102891290.330204516521901
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.401815 0.00241278
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.028 0.951 1.016 0.996 0.937
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1W9C X-ray 2.3Å A/B=707-1027.
2L1L NMR - B=504-630.
3GB8 X-ray 2.9Å A=1-1071.
4BSM X-ray 4.5Å A=1-1032.
4BSN X-ray 4.1Å A=1-1032.
5DIS X-ray 2.8Å A=5-1048.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 22190034
Rev cooperates with 25486595
Rev binds 10381392
11238447
19149558
1914955925486595
257231787543368
7745679
19149559
20972448
22783232
254865957634337
7637788
8633082
9070945
9368759
14554087
191495589368759
18508616
18976462
19149559
19342232
19535902
19536614
20953181
21613532
22421880
22675540
22783232
23082981
23754689
24606219
25486595
Pr55(Gag) exported by 10604476
22318151
24478440
matrix enhanced by 22114565
capsid upregulated by 22933280
Rev inhibited by 10875611
19020832
Rev exported by 12239324
22783232
matrix exported by 10604476
HIV-1 virus replication enhanced by expression of human gene 18976975
Tat interacts with 19454010
25496916
Pr55(Gag) enhanced by 19435492
2125116521251165
22318151
2527512522114565
Rev complexes with 25723178
Rev interacts with 10347184
19149559
1953590212134013
1648487
19535902
2161353222174317
24475978
25275125
9111043
9837918
10347184
10518602
18508616
19149558
19149559
19535902
19536614
22783232
23082981
25486594
25486595
255644439368759
16354571
17661632
18508616
19020832
19149558
19535902
20018238
20712566
20972448
21358275
22355797
22421880
22783232
23754689
24530126
Pr55(Gag) upregulates 22933280
22933280
Rev recruits 11836381
16354571
19535902
23082981
24158817
24255166
Rev enhanced by 12134013
20953181
Rev associates with 25589658
Rev regulated by 21364984
22114565
22114565
22783232
25275125
Vpr exported by 11152524
12805458
2001252912805458
20012529
Rev co-localizes with 15189895
24255166
25275125
Rev modulated by 12134041
19149558
19149559
19535902

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03008 Ribosome biogenesis in eukaryotes - Homo sapiens (human)
hsa03013 RNA transport - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
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