Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007888
UniProt IDO43516
Primary gene name(s)WIPF1
Synonym gene name(s)WASPIP, WIP
Protein nameWAS/WASL-interacting protein family member 1
Protein functionPlays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles, By similarity. Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus. {ECO:0000250, ECO:0000269|PubMed:10878810, ECO:0000269|PubMed:19910490, ECO:0000269|PubMed:9405671}.
Subcellular locationCytoplasmic vesicle {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell projection, ruffle {ECO:0000269|PubMed:19910490}. Note=Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43516
Gene Ontology
(Biological Process)
Complete annatation
actin filament-based movement [GO:0030048];
actin polymerization or depolymerization [GO:0008154];
endocytosis [GO:0006897];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
positive regulation of actin nucleation [GO:0051127];
protein complex assembly [GO:0006461];
regulation of cell shape [GO:0008360];
response to other organism [GO:0051707]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779];
actin filament binding [GO:0051015];
profilin binding [GO:0005522]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
actin filament [GO:0005884];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
ruffle [GO:0001726]
Protein-protein interaction113295
Phylogenetic treeO43516
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8343087035885770.01262919581774990.0318359657743982
AZA vs. DISU0.09045313632362480.7203868172825950.973374730783303
AZA vs. IL7-0.2304816822635680.2294718937240770.989557594204189
AZA vs. SAHA-0.3612830220830860.1391373292999490.474089006626801
DISU vs. CD30.9123399304460570.01301550859336690.0361451320074451
DISU vs. IL7-0.3302585400416680.189203425067530.563096804381579
DISU vs. SAHA-0.4500102527033470.1224192370179430.449451712137583
DMSO vs. AZA-0.04625107852579010.7814683638817091
DMSO vs. CD30.7777738953148560.01689974960729650.0388279286005675
DMSO vs. DISU-0.1382895189304660.57024879751820.929538591477994
DMSO vs. IL7-0.1770953470133970.323105365002970.799817716554907
DMSO vs. SAHA-0.3214954114456810.1726864617068410.50329871676345
HIV vs. Mock in Activation0.1357885868045230.8291098341865370.999983755607037
HIV vs. Mock in Latency0.1053559366857180.5211655098707620.999834320637052
IL7 vs. CD30.6107891976575630.06003498038094780.12743377823997
SAHA vs. CD30.4493231301346860.2061351200127020.308977149253175
SAHA vs. IL7-0.1335808544377170.5825129220485630.785867704764746
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.265094 0.0754543
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.995 1.08 0.981 1.049 1.103
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202665_s_at 1.49 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2A41 X-ray 2.6Å C=29-60.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)