Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007832
UniProt IDQ4G0F5
Primary gene name(s)VPS26B
Synonym gene name(s)unknown
Protein nameVacuolar protein sorting-associated protein 26B
Protein functionActs as component of the retromer cargo-selective complex, CSC. The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network, TGN and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. May be involved in retrograde transport of SORT1 but not of IGF2R. Acts redundantly with VSP26A in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1, By similarity. {ECO:0000250|UniProtKB:O75436, ECO:0000250|UniProtKB:Q8C0E2}.
Subcellular locationCytoplasm {ECO:0000250|UniProtKB:Q8C0E2}. Membrane;
Peripheral membrane protein {ECO:0000250|UniProtKB:Q8C0E2}. Early endosome {ECO:0000250|UniProtKB:Q8C0E2}. Late endosome {ECO:0000250|UniProtKB:Q8C0E2}. Note=Localizes to early and late endosomal structures, By similarity. {ECO:0000250|UniProtKB:Q8C0E2}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q4G0F5
Gene Ontology
(Biological Process)
Complete annatation
intracellular protein transport [GO:0006886];
regulation of macroautophagy [GO:0016241];
retrograde transport, endosome to Golgi [GO:0042147]
Gene Ontology
(Molecular Function)
Complete annatation
protein transporter activity [GO:0008565]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
early endosome [GO:0005769];
endosome [GO:0005768];
late endosome [GO:0005770];
retromer, cargo-selective complex [GO:0030906];
retromer complex [GO:0030904]
Protein-protein interaction125215
Phylogenetic treeQ4G0F5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.487532364772821.00138166009289e-056.17552608674993e-05
AZA vs. DISU0.07673492028988420.7629370724787440.976021571236163
AZA vs. IL7-0.1561356421857340.4171063282675590.999311006273513
AZA vs. SAHA0.01450595985096260.9525977626770990.990070051757834
DISU vs. CD31.552590619788752.79920962905633e-050.000183937602216195
DISU vs. IL7-0.2423154325862590.3379498709704560.718196532850226
DISU vs. SAHA-0.06043778702582570.8373932662483890.957937888454383
DMSO vs. AZA0.04917231425736460.7692091967762741
DMSO vs. CD31.523812406947573.59944203509599e-062.2908996307022e-05
DMSO vs. DISU-0.02978338591127580.9033080150951020.988283279918411
DMSO vs. IL7-0.1978576275896650.2715335954031250.769218388958909
DMSO vs. SAHA-0.04014261761960130.8648679392267920.964573919525684
HIV vs. Mock in Activation-0.06037956818416540.9227990354238540.999983755607037
HIV vs. Mock in Latency0.05117416158136740.7564402018392310.999834320637052
IL7 vs. CD31.338061960713414.56872733604063e-050.000293790218157596
SAHA vs. CD31.477901111888845.1747797291446e-050.000267893955747884
SAHA vs. IL70.1675902316606160.4922775571718470.722820281327394
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.442627 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 1.049 1.066 1.1 1.171
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)