Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007830
UniProt IDQ9BRG1
Primary gene name(s)VPS25
Synonym gene name(s)DERP9, EAP20
Protein nameVacuolar protein-sorting-associated protein 25
Protein functionComponent of the ESCRT-II complex, endosomal sorting complex required for transport II, which is required for multivesicular body, MVB formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. {ECO:0000269|PubMed:18723511}.
Subcellular locationCytoplasm. Endosome membrane. Nucleus, nucleoplasm. Note=Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BRG1
Gene Ontology
(Biological Process)
Complete annatation
autophagy [GO:0006914];
endosomal transport [GO:0016197];
multivesicular body assembly [GO:0036258];
protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [GO:0043328];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
protein homodimerization activity [GO:0042803];
protein N-terminus binding [GO:0047485];
structural molecule activity [GO:0005198]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endosome membrane [GO:0010008];
ESCRT II complex [GO:0000814];
extracellular exosome [GO:0070062];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction124039
Phylogenetic treeQ9BRG1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.248743622589710.0001809634059509730.000812233607651302
AZA vs. DISU-0.2153825879491730.4016001456326520.904620531271159
AZA vs. IL70.1227191386389560.5293657128502590.999311006273513
AZA vs. SAHA-0.2375660975044140.3351660777360690.707364705739203
DISU vs. CD3-1.476711565611788.47357933200232e-050.000490517414940872
DISU vs. IL70.3279576752670190.1976758498694270.572232260270726
DISU vs. SAHA-0.01943271187775160.9475567289110660.988308743958657
DMSO vs. AZA0.05931208780261080.72857451601481
DMSO vs. CD3-1.199898293396020.0002332678666698840.000956283592551225
DMSO vs. DISU0.2729729812446450.2687667473998940.787539351218434
DMSO vs. IL70.07047182204537050.6990862992961390.937788846060647
DMSO vs. SAHA-0.3023249196158430.2045411179274450.544952346883145
HIV vs. Mock in Activation-0.01648011441299550.9788875985929540.999983755607037
HIV vs. Mock in Latency0.07656259672479570.6481689844300230.999834320637052
IL7 vs. CD3-1.120401952787330.0006156449240826150.00280400096892865
SAHA vs. CD3-1.509561968883243.03659941472967e-050.000167686160424977
SAHA vs. IL7-0.3618810569361550.1414841580415710.354699327223517
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0699433 0.692372
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.007 0.937 0.993 1.109 1.039
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2ZME X-ray 2.9Å C/D=1-102.
3CUQ X-ray 2.6Å C/D=1-176.
3HTU X-ray 2.0Å A/C/E/G=102-176.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid decreases 26268989
capsid impairs 26268989
p2 impairs 26268989
capsid involves 26268989
HIV-1 virus replication enhanced by expression of human gene 26268989
Pr55(Gag) interacts with 21543492
Pr55(Gag) impairs 26268989

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)