Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007748
UniProt IDQ92890
Primary gene name(s)UFD1L
Synonym gene name(s)unknown
Protein nameUbiquitin fusion degradation protein 1 homolog
Protein functionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Subcellular locationNucleus {ECO:0000250}. Cytoplasm, cytosol {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q92890
Gene Ontology
(Biological Process)
Complete annatation
ER-associated misfolded protein catabolic process [GO:0071712];
error-free translesion synthesis [GO:0070987];
retrograde protein transport, ER to cytosol [GO:0030970];
skeletal system development [GO:0001501];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
thiol-dependent ubiquitin-specific protease activity [GO:0004843]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
UFD1-NPL4 complex [GO:0036501];
VCP-NPL4-UFD1 AAA ATPase complex [GO:0034098]
Protein-protein interaction113200
Phylogenetic treeQ92890
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5642167866642030.09133233878662760.163487055842286
AZA vs. DISU0.9283511372421330.000290974526659160.0332139530288099
AZA vs. IL70.1122940050706160.5603569588308990.999311006273513
AZA vs. SAHA0.1631397016397460.5064077794996650.824985503963898
DISU vs. CD30.349661203490370.3365359763794770.469801026756369
DISU vs. IL7-0.8248184870958540.001188284873126720.0309385051163823
DISU vs. SAHA-0.7628412252304380.01058193390588160.107321888220418
DMSO vs. AZA0.009225443421362010.9562924576641651
DMSO vs. CD3-0.5655195532765340.08234558462418410.145808207847124
DMSO vs. DISU-0.9206517151834960.0001914037904070520.0223601172204207
DMSO vs. IL70.1102483905412780.5410227053251030.896696737481162
DMSO vs. SAHA0.1473004497038310.5352406072903780.830529107054926
HIV vs. Mock in Activation0.04097820803551340.9474494533758840.999983755607037
HIV vs. Mock in Latency0.04996630841915440.7630700549291440.999834320637052
IL7 vs. CD3-0.4446482824587170.17327921117640.289624922384329
SAHA vs. CD3-0.425203792853770.2487187110418380.358061805063097
SAHA vs. IL70.0478909472621250.8452288988483790.934353366962412
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.465726 0.0108507
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.996 0.998 1.062 1.048 0.94
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2YUJ NMR - A=11-193.
5C1B X-ray 3.0Å U/V=221-241.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu cooperates with 20442859

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)