Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007734
UniProt IDQ9UHD9
Primary gene name(s)UBQLN2
Synonym gene name(s)N4BP4, PLIC2
Protein nameUbiquilin-2
Protein functionPlays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system, UPS, autophagy and the endoplasmic reticulum-associated protein degradation, ERAD pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding, via UBA domain to their polyubiquitin chains and by interacting, via ubiquitin-like domain with the subunits of the proteasome, PubMed:10983987. Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome, PubMed:24215460, PubMed:18307982. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion, PubMed:19148225, PubMed:20529957. Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits, CCPs, PubMed:18199683. {ECO:0000269|PubMed:10983987, ECO:0000269|PubMed:18199683, ECO:0000269|PubMed:18307982, ECO:0000269|PubMed:19148225, ECO:0000269|PubMed:20529957, ECO:0000269|PubMed:24215460}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:18199683}. Nucleus {ECO:0000269|PubMed:9853615}. Membrane {ECO:0000250|UniProtKB:Q9QZM0}. Cytoplasmic vesicle, autophagosome {ECO:0000269|PubMed:19148225}. Note=Colocalizes with a subset of proteasomes, namely those that are cytoskeleton associated or free in the cytosol. Associated with fibers in mitotic cells. {ECO:0000269|PubMed:10983987}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UHD9
Gene Ontology
(Biological Process)
Complete annatation
autophagy [GO:0006914];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
negative regulation of clathrin-dependent endocytosis [GO:1900186];
negative regulation of G-protein coupled receptor internalization [GO:1904021];
positive regulation of ER-associated ubiquitin-dependent protein catabolic process [GO:1903071];
regulation of autophagosome assembly [GO:2000785];
regulation of macroautophagy [GO:0016241]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome [GO:0005776];
cytoplasm [GO:0005737];
cytoplasmic vesicle [GO:0031410];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction119006
Phylogenetic treeQ9UHD9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8569244779000870.009729653498723010.0254681030796723
AZA vs. DISU-0.2362517040856770.3506945381416870.887534614463883
AZA vs. IL70.3315787823364360.08510326001502740.799241505019015
AZA vs. SAHA-0.1596889163871930.5130552224827250.82900572662714
DISU vs. CD30.6090744146133850.09533833726360540.179506632014937
DISU vs. IL70.55831584857830.02709612068402680.210163190859568
DISU vs. SAHA0.078490988136740.7876351846478860.942804474138073
DMSO vs. AZA-0.005295391579387360.9748339262964271
DMSO vs. CD30.8409680300036050.009307161626892820.0234468879446723
DMSO vs. DISU0.2293297482253140.3475913962473890.842220911587404
DMSO vs. IL70.3441703877499230.05602072614432730.463763012974044
DMSO vs. SAHA-0.1599926460133080.4976241571011970.812534525641619
HIV vs. Mock in Activation0.1523509025614640.8070487744441440.999983755607037
HIV vs. Mock in Latency0.05747808182164720.7276492961570830.999834320637052
IL7 vs. CD31.194685655623050.000254598703869680.00130907322086777
SAHA vs. CD30.6740835634056650.05847244638885510.112711661176777
SAHA vs. IL7-0.4932077964206520.04330630880816880.166648777361532
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0640665 0.716421
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.007 0.699 0.843 0.9 1.056
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1J8C NMR - A=1-103.
2NBV NMR - B=26-103.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)