Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007716
UniProt IDO14933
Primary gene name(s)UBE2L6
Synonym gene name(s)UBCH8
Protein nameUbiquitin/ISG15-conjugating enzyme E2 L6
Protein functionCatalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. {ECO:0000269|PubMed:15131269, ECO:0000269|PubMed:16428300, ECO:0000269|PubMed:20508617}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14933
Gene Ontology
(Biological Process)
Complete annatation
cellular protein metabolic process [GO:0044267];
cellular protein modification process [GO:0006464];
ISG15-protein conjugation [GO:0032020];
modification-dependent protein catabolic process [GO:0019941];
negative regulation of type I interferon production [GO:0032480];
translesion synthesis [GO:0019985]
Gene Ontology
(Molecular Function)
Complete annatation
ISG15 transferase activity [GO:0042296];
ubiquitin binding [GO:0043130];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
nucleoplasm [GO:0005654]
Protein-protein interaction114672
Phylogenetic treeO14933
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: 3.186916015; Folds changes 16h: 2.03353948; Tested: tested;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.116045916568860.000808326799641490.00301189160054303
AZA vs. DISU0.1223355317432590.6291050648662050.960057478314726
AZA vs. IL70.4160176473870160.03150219277099680.552773634036361
AZA vs. SAHA0.008037081168781660.973786371199330.993360541946713
DISU vs. CD3-1.005346376662310.006243504748000620.0195583105925522
DISU vs. IL70.2840697338186290.2611706064486620.646918648079785
DISU vs. SAHA-0.1119449954481410.7013233702206220.911254807534701
DMSO vs. AZA0.04249248771637040.800878393029631
DMSO vs. CD3-1.085096144936810.0008743715437499190.00304212639875116
DMSO vs. DISU-0.0818734863484210.7375960275806560.967699562672189
DMSO vs. IL70.3809681574897610.03495510050954380.38267838793128
DMSO vs. SAHA-0.03976659453290850.8662829787845160.965346995706017
HIV vs. Mock in Activation0.04784740381750720.939552007696470.999983755607037
HIV vs. Mock in Latency0.03556954036281790.8299758115480360.999834320637052
IL7 vs. CD3-0.6939720722880690.03411740795602750.0807556046044601
SAHA vs. CD3-1.131039643604580.001740118469045320.00574574612807031
SAHA vs. IL7-0.4098951556564770.0932862325863020.276257960134152
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.2 2.67E-05 1.5 0.004591423 1.9 0.003355488
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.42342 0.00132191
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.205 1.46E-04

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.873 1.008 0.922 0.902 1.012
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201649_at 1.42 Yes upregulated in CD4+ cells
201649_at 1.63 Yes upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1WZV X-ray 2.1Å A/B=2-153.
1WZW X-ray 2.4Å A=2-153.
2KJH NMR - A=2-153.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa05012 Parkinson's disease - Homo sapiens (human)