Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007663
UniProt IDP33981
Primary gene name(s)TTK
Synonym gene name(s)MPS1, MPS1L1
Protein nameDual specificity protein kinase TTK
Protein functionPhosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity, via direct CDCA8 phosphorylation at the centromere, and for the mitotic checkpoint. {ECO:0000269|PubMed:18243099}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P33981
Gene Ontology
(Biological Process)
Complete annatation
chromosome separation [GO:0051304];
female meiosis chromosome segregation [GO:0016321];
meiotic spindle assembly checkpoint [GO:0033316];
mitotic spindle assembly checkpoint [GO:0007094];
mitotic spindle organization [GO:0007052];
peptidyl-serine phosphorylation [GO:0018105];
peptidyl-threonine phosphorylation [GO:0018107];
positive regulation of cell proliferation [GO:0008284];
positive regulation of pathway-restricted SMAD protein phosphorylation [GO:0010862];
protein autophosphorylation [GO:0046777];
protein localization to kinetochore [GO:0034501];
protein localization to meiotic spindle midzone [GO:1903096];
spindle organization [GO:0007051]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein serine/threonine/tyrosine kinase activity [GO:0004712];
protein serine/threonine kinase activity [GO:0004674];
protein tyrosine kinase activity [GO:0004713]
Gene Ontology
(Cellular Component)
Complete annatation
membrane [GO:0016020];
spindle [GO:0005819]
Protein-protein interaction113123
Phylogenetic treeP33981
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5252396330534120.6986752554446780.784974539699008
AZA vs. DISU0.9037975394523640.258165865350010.839581352451412
AZA vs. IL70.266507430637930.7157537678882930.999311006273513
AZA vs. SAHA-0.6655645426393330.3187850056937040.689336562304818
DISU vs. CD30.3661386666691440.7827581034042720.850748509047293
DISU vs. IL7-0.64587278114170.4030117735397550.764226430756235
DISU vs. SAHA-1.570164289859090.03162327401192130.213497065378592
DMSO vs. AZA-0.1851333750491150.7809461779034141
DMSO vs. CD3-0.7285482383018840.5910976083399990.688539818266612
DMSO vs. DISU-1.093336052912620.1274730003573060.613198530512005
DMSO vs. IL70.4599045923145950.4684653654482560.877499426457811
DMSO vs. SAHA-0.4881678399997510.36821779219550.715993243386485
HIV vs. Mock in Activation-0.3358267164942010.8844291843511460.999983755607037
HIV vs. Mock in Latency0.01559417358818620.9695928619560920.999834320637052
IL7 vs. CD3-0.2470234609249870.854177986609120.906245146206748
SAHA vs. CD3-1.219161066256180.3824458605830960.499087211550681
SAHA vs. IL7-0.9374031397152750.142260903461050.355621265376408
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.4 0.001542137 1.5 0.077252255 1.7 0.07417002
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.584853 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.024 2.75E-04

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.068 1.29 1.286 1.088 1.099
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
204822_at 1.86 Yes upregulated in CD4+ cells
204822_at 4.39 Yes upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01782 2,6-Dihydroanthra/1,9-Cd/Pyrazol-6-One experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2X9E X-ray 3.1Å A=514-828.
2ZMC X-ray 3.1Å A=510-857.
2ZMD X-ray 2.8Å A=510-857.
3CEK X-ray 2.3Å A=519-808.
3DBQ X-ray 2.7Å A=515-857.
3GFW X-ray 2.7Å A=519-808.
3H9F X-ray 2.6Å A=519-808.
3HMN X-ray 2.7Å A=510-809.
3HMO X-ray 2.4Å A=510-809.
3HMP X-ray 2.3Å A=510-809.
3VQU X-ray 2.4Å A=516-820.
3W1F X-ray 2.7Å A=516-820.
3WYX X-ray 2.9Å A=516-820.
3WYY X-ray 3.0Å A=516-820.
3WZJ X-ray 2.7Å A=516-820.
3WZK X-ray 2.3Å A=516-820.
4B94 X-ray 2.2Å A/B/C/D=62-239.
4BHZ X-ray 2.8Å A=519-808.
4BI0 X-ray 2.8Å A=519-808.
4BI1 X-ray 2.7Å A=519-808.
4BI2 X-ray 3.1Å A=519-808.
4C4E X-ray 2.6Å A=519-808.
4C4F X-ray 2.3Å A=519-808.
4C4G X-ray 2.6Å A=519-808.
4C4H X-ray 2.8Å A=519-808.
4C4I X-ray 2.6Å A=519-808.
4C4J X-ray 2.5Å A=519-808.
4CV8 X-ray 3.0Å A=519-808.
4CV9 X-ray 2.5Å A=519-808.
4CVA X-ray 2.5Å A=519-808.
4D2S X-ray 2.5Å A=514-795.
4H7X X-ray 2.6Å A/B=55-210.
4H7Y X-ray 1.8Å A/B/C/D=55-210.
4JS8 X-ray 1.9Å A=515-795.
4JT3 X-ray 2.2Å A=515-795.
4O6L X-ray 2.3Å A/B=515-795.
4ZEG X-ray 2.3Å A=515-795.
5AP0 X-ray 2.1Å A=519-808.
5AP1 X-ray 2.0Å A=519-808.
5AP2 X-ray 2.8Å A=519-808.
5AP3 X-ray 2.7Å A=519-808.
5AP4 X-ray 2.8Å A=519-808.
5AP5 X-ray 2.8Å A=519-808.
5AP6 X-ray 2.1Å A=519-808.
5AP7 X-ray 2.4Å A=519-808.
5EH0 X-ray 2.1Å A=519-794.
5EHY X-ray 2.2Å A=519-808.
5EI2 X-ray 2.6Å A=519-808.
5EI6 X-ray 2.0Å A=519-808.
5EI8 X-ray 2.1Å A=519-753.
5LJJ X-ray 3.0Å A=519-808.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)