Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007639
UniProt IDQ99816
Primary gene name(s)TSG101
Synonym gene name(s)unknown
Protein nameTumor susceptibility gene 101 protein
Protein functionComponent of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies, MVBs. Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan, PubMed:22660413. {ECO:0000269|PubMed:11916981, ECO:0000269|PubMed:17556548, ECO:0000269|PubMed:17853893, ECO:0000269|PubMed:21070952, ECO:0000269|PubMed:21757351, ECO:0000269|PubMed:22660413}.
Subcellular locationCytoplasm. Membrane;
Peripheral membrane protein. Nucleus. Late endosome membrane;
Peripheral membrane protein. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99816
Gene Ontology
(Biological Process)
Complete annatation
autophagosome maturation [GO:0097352];
autophagy [GO:0006914];
cell cycle arrest [GO:0007050];
cell division [GO:0051301];
endosomal transport [GO:0016197];
endosome to lysosome transport [GO:0008333];
exosomal secretion [GO:1990182];
intracellular transport of virus [GO:0075733];
keratinocyte differentiation [GO:0030216];
multivesicular body assembly [GO:0036258];
negative regulation of cell proliferation [GO:0008285];
negative regulation of epidermal growth factor receptor signaling pathway [GO:0042059];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of exosomal secretion [GO:1903543];
positive regulation of ubiquitin-dependent endocytosis [GO:2000397];
positive regulation of viral budding via host ESCRT complex [GO:1903774];
positive regulation of viral process [GO:0048524];
positive regulation of viral release from host cell [GO:1902188];
protein monoubiquitination [GO:0006513];
protein transport [GO:0015031];
regulation of cell growth [GO:0001558];
regulation of extracellular exosome assembly [GO:1903551];
regulation of MAP kinase activity [GO:0043405];
regulation of viral budding via host ESCRT complex [GO:1903772];
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [GO:0043162];
viral budding [GO:0046755];
viral budding via host ESCRT complex [GO:0039702];
viral life cycle [GO:0019058]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-dependent protein binding [GO:0048306];
DNA binding [GO:0003677];
ligand-dependent nuclear receptor transcription coactivator activity [GO:0030374];
protein homodimerization activity [GO:0042803];
transcription corepressor activity [GO:0003714];
ubiquitin binding [GO:0043130];
ubiquitin protein ligase binding [GO:0031625];
virion binding [GO:0046790]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
early endosome [GO:0005769];
endosome [GO:0005768];
endosome membrane [GO:0010008];
ESCRT I complex [GO:0000813];
extracellular exosome [GO:0070062];
late endosome [GO:0005770];
late endosome membrane [GO:0031902];
multivesicular body [GO:0005771];
nucleolus [GO:0005730];
plasma membrane [GO:0005886]
Protein-protein interaction113102
Phylogenetic treeQ99816
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4610253299493640.1656100538864120.263549227344103
AZA vs. DISU0.1373752235850650.5885116971116640.955158276076142
AZA vs. IL70.1431274139134150.4601653910111150.999311006273513
AZA vs. SAHA-0.2326177524216980.3436738432316110.715741850355528
DISU vs. CD3-0.335876301069570.3626859120133380.496695506285914
DISU vs. IL7-0.003825481962748710.987935099253560.998667043602724
DISU vs. SAHA-0.3680964150561030.2095023358848610.583332742324224
DMSO vs. AZA-0.006961960604941030.9672219137655321
DMSO vs. CD3-0.4781137366203490.140963501745970.225987909733941
DMSO vs. DISU-0.1458494530979910.5514808458653710.923184551298667
DMSO vs. IL70.1570444330905810.3863936856262110.837514642401658
DMSO vs. SAHA-0.2319664211169850.3283978503415720.6821908490542
HIV vs. Mock in Activation-0.1743467193367060.7793877563685180.999983755607037
HIV vs. Mock in Latency0.04969390219069920.7653336281107990.999834320637052
IL7 vs. CD3-0.3109023310930310.3377077715589510.475740280057167
SAHA vs. CD3-0.7172864167200350.04478312531282240.090612472940955
SAHA vs. IL7-0.3782975226123520.1225144281326770.324495704944366
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0557739 0.763869
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.98 0.83 0.789 0.78 0.84
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1KPP NMR - A=1-145.
1KPQ NMR - A=1-145.
1M4P NMR - A=1-145.
1M4Q NMR - A=1-145.
1S1Q X-ray 2.0Å A/C=1-145.
2F0R X-ray 2.2Å A/B=1-145.
3IV1 X-ray 2.5Å A/B/C/D/E/F/G/H=229-304.
3OBQ X-ray 1.4Å A=2-145.
3OBS X-ray 1.5Å A=2-145.
3OBU X-ray 1.6Å A=2-145.
3OBX X-ray 1.6Å A=2-145.
3P9G X-ray 1.8Å A=2-145.
3P9H X-ray 1.8Å A=2-145.
4EJE X-ray 2.2Å A/B=1-145.
4YC1 X-ray 2.0Å A/B/C=1-145.
4ZNY X-ray 2.4Å A=4-145.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
p6 complexes with 17982468
18005716
19064259
19282983
19802344
20088757
p6 interacts with 18267010
18321968
19143627
20088757
2200403519053244
19099395
Pr55(Gag) recruits 19282983
20088757
2275464925488808
p6 binds 11427703
11595185
11726971
11805336
12006492
12379843
12388682
12598123
12663786
12743307
12915533
14581576
15795524
16138902
16234236
16470130
16571793
16808324
16940516
17556548
17942528
18267010
18789977
19020832
19053244
19064259
19099395
19143627
19244744
19282983
19802344
20088757
21070952
21110817
21152581
21159863
21643473
22004035
22421880
2326627912900394
16470130
17083721
19099395
19143627
19282983
2107095214505569
14505570
14519844
16470130
17982468
19020832
19064259
19802344
20088757
21110817
22004035
22421880
23266279
2389534516234236
19914066
21070952
Nef interacts with 25919665
capsid impairs 26268989
p2 interacts with 20426868
nucleocapsid recruits 25488808
25749978
Nef associates with 23317503
Pr55(Gag) co-localizes with 24436186
25488808
25710462
p2 impairs 26268989
reverse transcriptase inhibited by 25488808
capsid affects 25973004
HIV-1 virus replication enhnaced by expression of human gene 26268989
HIV-1 virus replication enhanced by expression of human gene 18976975
2563397725973004
Pr55(Gag) interacts with 18267010
18367816
2008875719053244
1909939520427536
23408603
23895345
24436186
25099357
25710462
Nef enhances release of 26109641
Pr55(Gag) binds 11427703
11595185
11726971
11805336
12006492
12379843
12388682
12598123
12663786
12743307
12915533
14581576
15168195
16138902
16470130
16571793
16808324
17556548
18267010
18367816
18789977
19053244
19064259
19099395
19143627
19244744
19282983
19401538
19802344
20088757
21070952
21152581
21159863
21505419
21643473
21666754
21762796
21762798
21875593
22004035
22291694
22421880
22754649
22761998
2330548612900394
16470130
19099395
19143627
19282983
20088757
2107095214505569
14505570
14519844
16470130
19064259
19401538
19802344
20012524
20088757
20427536
21841072
22004035
22421880
22761998
2333071921070952
21875593
Vpu requires 26317613
Pr55(Gag) regulated by 15218037
16940516
17083721
19064259
20012524
20018238
20088757
20712566
21762796
21762798
22004035
22754649
23027949
24107264
25099357
2574997815256501
16808324
20088757
Pr55(Gag) impairs 26268989

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
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