Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007609
UniProt IDQ13049
Primary gene name(s)TRIM32
Synonym gene name(s)HT2A
Protein nameE3 ubiquitin-protein ligase TRIM32
Protein functionHas an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1, dysbindin and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. {ECO:0000269|PubMed:19349376, ECO:0000269|PubMed:22500027}.
Subcellular locationCytoplasm. Note=Localized in cytoplasmic bodies, often located around the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13049
Gene Ontology
(Biological Process)
Complete annatation
actin ubiquitination [GO:0007014];
axon development [GO:0061564];
fat cell differentiation [GO:0045444];
innate immune response [GO:0045087];
muscle cell cellular homeostasis [GO:0046716];
negative regulation of fibroblast proliferation [GO:0048147];
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [GO:1902230];
negative regulation of viral release from host cell [GO:1902187];
negative regulation of viral transcription [GO:0032897];
positive regulation of cell cycle [GO:0045787];
positive regulation of cell growth [GO:0030307];
positive regulation of cell migration [GO:0030335];
positive regulation of cell motility [GO:2000147];
positive regulation of chemokine, C-C motif ligand 20 production [GO:1903886];
positive regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043123];
positive regulation of interleukin-17-mediated signaling pathway [GO:1903883];
positive regulation of neurogenesis [GO:0050769];
positive regulation of neuron differentiation [GO:0045666];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
positive regulation of protein catabolic process [GO:0045732];
positive regulation of proteolysis [GO:0045862];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
positive regulation of striated muscle cell differentiation [GO:0051155];
positive regulation of tumor necrosis factor-mediated signaling pathway [GO:1903265];
protein polyubiquitination [GO:0000209];
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of type I interferon production [GO:0032479];
response to tumor necrosis factor [GO:0034612];
response to UV [GO:0009411];
tissue homeostasis [GO:0001894]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
myosin binding [GO:0017022];
protein self-association [GO:0043621];
RNA binding [GO:0003723];
Tat protein binding [GO:0030957];
transcription coactivator activity [GO:0003713];
translation initiation factor binding [GO:0031369];
ubiquitin binding [GO:0043130];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634];
striated muscle myosin thick filament [GO:0005863]
Protein-protein interaction116608
Phylogenetic treeQ13049
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8592158540551720.009685689068538730.0253706474871421
AZA vs. DISU-0.1842163878576010.4699619842170840.926405371176645
AZA vs. IL70.006829197314616740.9720637978780710.999311006273513
AZA vs. SAHA-0.07910199632675210.7480309661212060.930952123332784
DISU vs. CD30.6631089246798230.06989536161380650.140837144895939
DISU vs. IL70.1813522459798330.4748412367545660.810714150984736
DISU vs. SAHA0.1071532121427720.7146107134421880.915550590566598
DMSO vs. AZA-0.03843021826003970.8220261007974161
DMSO vs. CD30.8086133658649510.01262921526320480.0304268555797506
DMSO vs. DISU0.1436481180237120.5591321078043060.92493094013292
DMSO vs. IL70.05254689223853640.7734924463077140.953700815775867
DMSO vs. SAHA-0.04593882312064270.8469131855459180.959461020163348
HIV vs. Mock in Activation0.02540139070530060.9676243148025680.999983755607037
HIV vs. Mock in Latency0.02889857636591710.8633979955289840.999834320637052
IL7 vs. CD30.8723666021837030.007341204868333560.0231542123834258
SAHA vs. CD30.7561643921407660.03416451836817650.0724134025047003
SAHA vs. IL7-0.08805051252637510.7198351661207530.871060070169723
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.816022 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.991 0.978 1.047 1.069 0.977
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CT2 NMR - A=10-84.
5FEY X-ray 2.2Å A/B=7-93.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat binds 7778269

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)