Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007585
UniProt IDQ15628
Primary gene name(s)TRADD
Synonym gene name(s)unknown
Protein nameTumor necrosis factor receptor type 1-associated DEATH domain protein
Protein functionThe nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A, By similarity. Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. {ECO:0000250}.
Subcellular locationNucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:11684708}. Note=Shuttles between the cytoplasm and the nucleus. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15628
Gene Ontology
(Biological Process)
Complete annatation
activation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0006919];
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [GO:0097296];
apoptotic process [GO:0006915];
death-inducing signaling complex assembly [GO:0071550];
extrinsic apoptotic signaling pathway [GO:0097191];
extrinsic apoptotic signaling pathway via death domain receptors [GO:0008625];
I-kappaB kinase/NF-kappaB signaling [GO:0007249];
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [GO:1902042];
positive regulation of apoptotic process [GO:0043065];
positive regulation of hair follicle development [GO:0051798];
positive regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043123];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
protein heterooligomerization [GO:0051291];
regulation of extrinsic apoptotic signaling pathway via death domain receptors [GO:1902041];
regulation of necrotic cell death [GO:0010939];
regulation of tumor necrosis factor-mediated signaling pathway [GO:0010803];
signal transduction [GO:0007165];
tumor necrosis factor-mediated signaling pathway [GO:0033209]
Gene Ontology
(Molecular Function)
Complete annatation
binding, bridging [GO:0060090];
death domain binding [GO:0070513];
identical protein binding [GO:0042802];
kinase binding [GO:0019900];
signal transducer activity [GO:0004871]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
death-inducing signaling complex [GO:0031264];
membrane raft [GO:0045121];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
receptor complex [GO:0043235]
Protein-protein interaction114257
Phylogenetic treeQ15628
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-3.181767027100871.86344939479e-103.09998998656455e-09
AZA vs. DISU-0.3032215069311810.2319666383111520.818071474852885
AZA vs. IL7-0.105602131841340.615097871752890.999311006273513
AZA vs. SAHA-0.1814377759970810.4574469338081010.79769021597318
DISU vs. CD32.866644976341243.68175898657341e-085.00892012306928e-07
DISU vs. IL70.1874062635575170.4579639806593640.800667218287658
DISU vs. SAHA0.1247911636118380.6693893873549420.899301787914632
DMSO vs. AZA-0.08040122420249840.7045337331372281
DMSO vs. CD33.094759196774211.33819266778801e-091.68984681812459e-08
DMSO vs. DISU0.2222663704192270.3848855267440390.858148378167284
DMSO vs. IL7-0.01851244201073630.9326274864772020.98672467966686
DMSO vs. SAHA-0.1072522423444710.6494626620309130.887621949322553
HIV vs. Mock in Activation0.2440336806261720.8085135810826890.999983755607037
HIV vs. Mock in Latency0.001047735560480860.9949486733414610.999834320637052
IL7 vs. CD33.080683384955896.32985330817348e-101.17067336090919e-08
SAHA vs. CD32.978197413630454.08799172291197e-095.9293585432419e-08
SAHA vs. IL7-0.07747933355376230.7504871266344940.887862460642147
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.179759 0.221609
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.397 1.405 2.03 1.179 1.195
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1F2H NMR - A=1-169.
1F3V X-ray 2.0Å A=1-179.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 upregulates 15103018

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04064 NF-kappa B signaling pathway - Homo sapiens (human)
hsa04071 Sphingolipid signaling pathway - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04622 RIG-I-like receptor signaling pathway - Homo sapiens (human)
hsa04657 IL-17 signaling pathway - Homo sapiens (human)
hsa04668 TNF signaling pathway - Homo sapiens (human)
hsa04920 Adipocytokine signaling pathway - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05160 Hepatitis C - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)